Biochemical and functional analysis of a citrus sinensis protein, CsEIE, involved ...
| Full text | |
| Author(s): |
de Lima Silva, Caio Cesar
[1]
;
Shimo, Hugo Massayoshi
[1]
;
de Felicio, Rafael
[1]
;
Mercaldi, Gustavo Fernando
[1]
;
Rocco, Silvana Aparecida
[1]
;
Benedetti, Celso Eduardo
[1]
Total Authors: 6
|
| Affiliation: | [1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083100 Campinas, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 9, MAR 7 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Salicylic acid (SA) and its methyl ester, methyl salicylate (MeSA) are well known inducers of local and systemic plant defense responses, respectively. MeSA is a major mediator of systemic acquired resistance (SAR) and its conversion back into SA is thought to be required for SAR. In many plant species, conversion of MeSA into SA is mediated by MeSA esterases of the SABP2 family. Here we show that the Citrus sinensis SABP2 homologue protein CsMES1 catalyzes the hydrolysis of MeSA into SA. Molecular modeling studies suggest that CsMES1 shares the same structure and SA-binding mode with tobacco SABP2. However, an amino acid polymorphism in the active site of CsMES1-related proteins suggested an important role in enzyme regulation. We present evidence that the side chain of this polymorphic residue directly influences enzyme activity and SA binding affinity in CsMES proteins. We also show that SA and CsMES1 transcripts preferentially accumulate during the incompatible interaction between Xanthomonas aurantifolii pathotype C and sweet orange plants. Moreover, we demonstrate that SA and MeSA inhibited citrus canker caused by Xanthomonas citri, whereas an inhibitor of CsMES1 enhanced canker formation, suggesting that CsMES1 and SA play a role in the local defense against citrus canker bacteria. (AU) | |
| FAPESP's process: | 13/22399-2 - Biochemical and functional analysis of a citrus sinensis protein, CsEIE, involved in the defense against Xanthomonas citri |
| Grantee: | Caio Cesar de Lima Silva |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 13/22507-0 - Functional characterization of CsWRKY, a sweet orange (citrus sinensis) transcription factor involved in citrus canker resistance |
| Grantee: | Hugo Massayoshi Shimo |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 16/03151-8 - Aminoacil-tRNA synthetases as targets for development of Agrobactericides intented for control of plant diseases caused by Xanthomonas species |
| Grantee: | Gustavo Fernando Mercaldi |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 11/20468-1 - Molecular mechanisms involved in pathogen adaptation and virulence, host resistance and symptom development in citrus-bacteria interactions |
| Grantee: | Celso Eduardo Benedetti |
| Support type: | Research Projects - Thematic Grants |