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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mitochondrial alternative oxidase is determinant for growth and sporulation in the early diverging fungus Blastocladiella emersonii

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Author(s):
Luevano-Martinez, Luis Alberto [1, 2] ; Caldeira da Silva, Camille C. [2] ; Nicastro, Gianlucca G. [2] ; Schumacher, I, Robert ; Kowaltowski, Alicia J. [3] ; Gomes, Suely L. [3]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[3] Schumacher, Robert, I, Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 123, n. 1, p. 59-65, JAN 2019.
Web of Science Citations: 0
Abstract

Blastocladiella emersonii is an early diverging fungus of the phylum Blastocladiomycota. During the life cycle of the fungus, mitochondrial morphology changes significantly, from a fragmented form in sessile vegetative cells to a fused network in motile zoospores. In this study, we visualize these morphological changes using a mitochondrial fluorescent probe and show that the respiratory capacity in zoospores is much higher than in vegetative cells, suggesting that mitochondrial morphology could be related to the differences in oxygen consumption. While studying the respiratory chain of the fungus, we observed an antimycin A and cyanide-insensitive, salicylhydroxamic (SHAM)-sensitive respiratory activity, indicative of a mitochondrial alternative oxidase (AOX) activity. The presence of AOX was confirmed by the finding of a B. emersonii cDNA encoding a putative AOX, and by detection of AOX protein in immunoblots. Inhibition of AOX activity by SHAM was found to significantly alter the capacity of the fungus to grow and sporulate, indicating that AOX participates in life cycle control in B. emersonii. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/12999-0 - Evolution of the cardiolipin biosynthetic pathway: biochemical implications for the evolution of the Eukarya domain
Grantee:Luis Alberto Luevano Martinez
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/19960-1 - Molecular aspects of the biology of the aquatic fungus Blastocladiella emersonii
Grantee:Suely Lopes Gomes
Support type: Regular Research Grants