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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oxidative stress induced by self-adhesive resin cements affects gene expression, cellular proliferation and mineralization potential of the MDPC-23 odontoblast-like cells

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Palacio Alvarez, Marcela Maciel [1] ; de Carvalho, Rafael Guzella [1] ; de Arruda Barbosa, Silvana Coelho [2] ; Polassi, Mackeler Ramos [2] ; Nascimento, Fabio Dupart [3] ; Perlatti D'Alpino, Paulo Henrique [2] ; dos Santos Tersariol, Ivarne Luis [1]
Total Authors: 7
[1] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil
[2] Univ Anhanguera Sao Paulo UNIAN SP, Biotechnol & Innovat Hlth Program, Sao Paulo, SP - Brazil
[3] Univ Mogi das Cruzes, Interdisciplinary Ctr Biochem Invest, Mogi Das Cruzes, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Dental Materials; v. 35, n. 4, p. 606-616, APR 2019.
Web of Science Citations: 0

Objective. Clinical issues have been raised about problems related to cytotoxic effects caused when applying self-adhesive cement. It was hypothesized that byproducts eluted from self-adhesive cements modulate oxidative stress response, the gene expression of signaling pathways of inflammatory process/transcriptional activators, and the expression and activity of interstitial collagenases, and modify the phenotypic characteristics of cellular proliferation and mineral deposition in odontoblastic-like cells. Methods. Cements (MaxCem Elite {[}MAX] and RelyX U200 {[}U200)]) were mixed, dispensed into moulds, and photoactivated according to the manufacturers' instructions. Immortalized rat odontoblast-like cells (MDPC-23) were cultured and exposed to polymerized specimens of cements for 4 h. Reactive oxidative specimen production and quantification of gene expression were evaluated. Cell proliferation assay and alizarin red staining were also performed to evaluate the disturbance induced by the cements on cellular proliferation and mineralization. Results. Despite their cytotoxic effects, both self-adhesive cements influenced the metabolism in the odontoblast cells on different scales. MAX induced significantly higher oxidative stress in odontoblast cells than U200. Gene expression varied as a function of exposure to self-adhesive cements; MAX induced the expression of pro-inflammatory cytokines such as TNF-alpha, whereas U200 downregulated, virtually depleted TNF-alpha expression, also inducing overexpression of the transcriptional factor Runx2. Overexpression of hemeoxygenase-1 (HO-1) and thioredoxin reductase 1 (TRXR1) occurred after exposure to both cements, antioxidant genes that are downstream of Keap1-Nrf2-ARE system. MAX significantly induced the overexpression of collagenase MMP-1, and U200 induced the expression of gelatinase MMP-2. MAX significantly inhibited cell proliferation whereas U200 significantly activated cell proliferation. Alizarin red staining revealed significantly decreased mineral deposition especially when exposed to MAX. Significance. These results support the hypothesis that byproducts of different self-adhesive cements play important roles in the highly orchestrated process which ultimately affect the cellular proliferation and the mineral deposition in odontoblastic-like cells, possibly delaying the reparative dentin formation after cementation of indirect restorations, especially on recently exposed dentin preparations. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/05822-9 - Proteinase-Activated Receptors (PARs) in the dentin-pulp complex: identification, modulation and signal tranduction in caries disease
Grantee:Fábio Dupart Nascimento
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/03964-6 - Glycosaminoglycans and proteoglycans: interplay between structure and function
Grantee:Helena Bonciani Nader
Support type: Research Projects - Thematic Grants