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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insulin action on protein synthesis and its association with eIF5A expression and hypusination

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Gomes de Proenca, Andre Ricardo [1] ; Pereira, Karina Danielle [1, 2] ; Meneguello, Leticia [1, 2] ; Tamborlin, Leticia [1, 2] ; Luchessi, Augusto Ducati [1, 2, 3]
Total Authors: 5
[1] Univ Campinas UNICAMP, Sch Appl Sci, Lab Biotechnol, Limeira, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Rio Claro, SP - Brazil
[3] Univ Estadual Campinas, Fac Ciencias Aplicadas, Lab Biotecnol, Rua Pedro Zaccaria, BR-13484350 Limeira, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MOLECULAR BIOLOGY REPORTS; v. 46, n. 1, p. 587-596, FEB 2019.
Web of Science Citations: 1

The hormone insulin plays a central role in the metabolism of carbohydrates, lipids, and proteins. In relation to protein metabolism, insulin stimulates amino acid uptake and activates protein synthesis in responsive cells by modulation of signal transduction pathways, such as associated to Akt/PkB, mTOR, S6Ks, 4E-BP1, and several translation initiation/elongation factors. In this context, there is no information on direct cellular treatment with insulin and effects on eukaryotic translation initiation factor 5A (eIF5A) regulation. The eIF5A protein contains an exclusive amino acid residue denominated hypusine, which is essential for its activity and synthesized by posttranslational modification of a specific lysine residue using spermidine as substrate. The eIF5A protein is involved in cellular proliferation and differentiation processes, as observed for satellite cells derived from rat muscles, revealing that eIF5A has an important role in muscle regeneration. The aim of this study was to determine whether eIF5A expression and hypusination are influenced by direct treatment of insulin on L6 myoblast cells. We observed that insulin increased the content of eIF5A transcripts. This effect occurred in cells treated or depleted of fetal bovine serum, revealing a positive insulin effect independent of other serum components. In addition, it was observed that hypusination follows the maintenance of eIF5A protein content in the serum depleted cells and treated with insulin. These results demonstrate that eIF5A is modulated by insulin, contributing the protein synthesis machinery control, as observed by puromycin incorporation in nascent proteins. (AU)

FAPESP's process: 10/18095-0 - Study of the eukaryotic translation initiation factor 5A (eIF5A) in mammals
Grantee:Augusto Ducati Luchessi
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 17/21914-1 - Functional characterization of the amino acid hypusine
Grantee:Leticia Tamborlin
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/20504-3 - Adjustment of polyamine contents by melatonin and effects in control of protein synthesis and degradation
Grantee:André Ricardo Gomes de Proença
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/27154-0 - Evaluation of eukaryotic translation initiation factor 5A (eIF5A) in the translation of S6Ks proteins
Grantee:Letícia Meneguello
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/23620-4 - Functional characterization of the translation factor eIF5A isoform a
Grantee:Karina Danielle Pereira
Support type: Scholarships in Brazil - Doctorate