Advanced search
Start date
Betweenand

Adjustment of polyamine contents by melatonin and effects in control of protein synthesis and degradation

Grant number: 13/20504-3
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2014
Effective date (End): January 31, 2017
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Augusto Ducati Luchessi
Grantee:André Ricardo Gomes de Proença
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

Over the past 20 years, the actions of the pineal gland and, consequently, melatonin has been widely studied. Melatonin is an amphiphilic molecule, a characteristic that allows it to reach several tissues of the body. There are few studies that have sought to investigate the effects of treatment with exogenous melatonin and the repercussions in striated muscle tissue, even less was shown on the acute and chronic effects of melatonin in the polyamine pathway. These molecules are involved in a variety of biological processes, including protein synthesis and cell differentiation. In this project, we hope to obtain results that allow us to assess the effects of the use of exogenous melatonin in muscle development, since, although studies have shown beneficial effects in other cell types (antioxidant, neuroprotective, permissive actions to other hormones), not yet it is clear if chronic melatonin use in supraphysiological doses can be beneficial to develop muscle and may even be used to treat muscular dystrophy cases. We aim to search changes resulting from treatment with melatonin in the complete set of transcripts (transcriptome) and study the effects of melatonin in the process of protein synthesis and degradation, as well as the concentrations of polyamines (putrescine, spermidine and spermine) with emphasis on modulation of expression and activity of eukaryotic translation initiation factor 5A (eIF-5A) in cell line (L6) and in skeletal muscle.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOMES DE PROENCA, ANDRE RICARDO; PEREIRA, KARINA DANIELLE; MENEGUELLO, LETICIA; TAMBORLIN, LETICIA; LUCHESSI, AUGUSTO DUCATI. Insulin action on protein synthesis and its association with eIF5A expression and hypusination. MOLECULAR BIOLOGY REPORTS, v. 46, n. 1, p. 587-596, FEB 2019. Web of Science Citations: 1.
PEREIRA, KARINA DANIELLE; TAMBORLIN, LETICIA; MENEGUELLO, LETICIA; GOMES DE PROENCA, ANDRE RICARDO; DE PAULA ANDRADE ALMEIDA, ISADORA CRISTINA; LOURENCO, ROGERIO FERREIRA; LUCHESSI, AUGUSTO DUCATI. Alternative Start Codon Connects eIF5A to Mitochondria. Journal of Cellular Physiology, v. 231, n. 12, p. 2682-2689, DEC 2016. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.

Filed patent(s) as a result of this research project

DISPOSITIVO SELETOR E TEMPORIZADOR PROGRAMÁVEL DE DISPONIBILIDADE DE LÍQUIDOS OU SÓLIDOS PARA ANIMAIS E USOS DO MESMO BR1020150314035 - Universidade Estadual de Campinas (UNICAMP) . André Ricardo Gomes De Proença; Augusto Ducati Luchessi - December 2015, 15