Identification and characterization of a homologue of human eIF5A isoform a in mouse
| Full text | |
| Author(s): |
Pereira, Karina Danielle
;
Tamborlin, Leticia
;
Meneguello, Leticia
;
Gomes De Proenca, Andre Ricardo
;
De Paula Andrade Almeida, Isadora Cristina
;
Lourenco, Rogerio Ferreira
;
Luchessi, Augusto Ducati
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Journal of Cellular Physiology; v. 231, n. 12, p. 2682-2689, DEC 2016. |
| Web of Science Citations: | 1 |
| Abstract | |
Eukaryotic translation initiation factor 5A (eIF5A), a protein containing the amino acid residue hypusine required for its activity, is involved in a number of physiological and pathological cellular processes. In humans, several EIF5A1 transcript variants encode the canonical eIF5A1 isoform B, whereas the hitherto uncharacterized variant A is expected to code for a hypothetical eIF5A1 isoform, referred to as isoform A, which has an additional N-terminal extension. Herein, we validate the existence of eIF5A1 isoform A and its production from transcript variant A. In fact, variant A was shown to encode both eIF5A1 isoforms A and B. Mutagenic assays revealed different efficiencies in the start codons present in variant A, contributing to the production of isoform B at higher levels than isoform A. Immunoblotting and mass spectrometric analyses showed that isoform A can undergo hypusination and acetylation at specific lysine residues, as observed for isoform B. Examination of the N-terminal extension suggested that it might confer mitochondrial targeting. Correspondingly, we found that isoform A, but not isoform B, co-purified with mitochondria when the proteins were overproduced. These findings suggest that eIF5A1 isoform A has a role in mitochondrial function. (C) 2016 Wiley Periodicals, Inc. (AU) | |
| FAPESP's process: | 13/20504-3 - Adjustment of polyamine contents by melatonin and effects in control of protein synthesis and degradation |
| Grantee: | André Ricardo Gomes de Proença |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 14/01386-2 - The role of eukaryotic translation initiation factor 5A (eIF5A) in the translation of S6Ks proteins |
| Grantee: | Letícia Meneguello |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 10/18095-0 - Study of the eukaryotic translation initiation factor 5A (eIF5A) in mammals |
| Grantee: | Augusto Ducati Luchessi |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/25263-4 - Identification and characterization of a homologue of human eIF5A isoform a in mouse |
| Grantee: | Isadora Cristina de Paula Andrade Almeida |
| Support type: | Scholarships in Brazil - Scientific Initiation |