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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alternative Start Codon Connects eIF5A to Mitochondria

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Pereira, Karina Danielle ; Tamborlin, Leticia ; Meneguello, Leticia ; Gomes De Proenca, Andre Ricardo ; De Paula Andrade Almeida, Isadora Cristina ; Lourenco, Rogerio Ferreira ; Luchessi, Augusto Ducati
Total Authors: 7
Document type: Journal article
Source: Journal of Cellular Physiology; v. 231, n. 12, p. 2682-2689, DEC 2016.
Web of Science Citations: 1

Eukaryotic translation initiation factor 5A (eIF5A), a protein containing the amino acid residue hypusine required for its activity, is involved in a number of physiological and pathological cellular processes. In humans, several EIF5A1 transcript variants encode the canonical eIF5A1 isoform B, whereas the hitherto uncharacterized variant A is expected to code for a hypothetical eIF5A1 isoform, referred to as isoform A, which has an additional N-terminal extension. Herein, we validate the existence of eIF5A1 isoform A and its production from transcript variant A. In fact, variant A was shown to encode both eIF5A1 isoforms A and B. Mutagenic assays revealed different efficiencies in the start codons present in variant A, contributing to the production of isoform B at higher levels than isoform A. Immunoblotting and mass spectrometric analyses showed that isoform A can undergo hypusination and acetylation at specific lysine residues, as observed for isoform B. Examination of the N-terminal extension suggested that it might confer mitochondrial targeting. Correspondingly, we found that isoform A, but not isoform B, co-purified with mitochondria when the proteins were overproduced. These findings suggest that eIF5A1 isoform A has a role in mitochondrial function. (C) 2016 Wiley Periodicals, Inc. (AU)

FAPESP's process: 13/20504-3 - Adjustment of polyamine contents by melatonin and effects in control of protein synthesis and degradation
Grantee:André Ricardo Gomes de Proença
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/01386-2 - The role of eukaryotic translation initiation factor 5A (eIF5A) in the translation of S6Ks proteins
Grantee:Letícia Meneguello
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/18095-0 - Study of the eukaryotic translation initiation factor 5A (eIF5A) in mammals
Grantee:Augusto Ducati Luchessi
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/25263-4 - Identification and characterization of a homologue of human eIF5A isoform a in mouse
Grantee:Isadora Cristina de Paula Andrade Almeida
Support type: Scholarships in Brazil - Scientific Initiation