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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Screening of Lipopeptide-Producing Strains of Bacillus sp. Using a New Automated and Sensitive Fluorescence Detection Method

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Author(s):
Heuson, Egon [1] ; Etchegaray, Augusto [2] ; Filipe, Stephanie L. [1] ; Beretta, Daniel [2] ; Chevalier, Mickael [1] ; Phalip, Vincent [1] ; Coutte, Francois [1]
Total Authors: 7
Affiliation:
[1] Univ Littoral Cote dOpale, Univ Artois, Univ Lille, INRA, ISA, EA 7394, ICV Inst Charles Viollette, F-59000 Lille - France
[2] Pontifical Catholic Univ Campinas, Fac Chem, Rod D Pedro 1, Km 136, BR-13086900 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Biotechnology Journal; v. 14, n. 4 APR 2019.
Web of Science Citations: 0
Abstract

Lipopeptides, such as surfactins are important biosurfactants produced by Bacillus sp. that find applications in many areas (environment, medicine, and food industries). Giving their importance, the use of simple detection methods will facilitate screening and quantification. In the present work, the authors describe a completely automated workflow for the screening of lipopeptide-producing strains, including quantification. First, isolated colonies from environmental samples are automatically picked and inoculated in 96 wells growth plate. After overnight incubation, surfactin produced in the broth is quantified, using a new sensitive fluorescent method. The method uses fluorescein (FL), which is an anionic dye at neutral to alkaline pH and forms a stable complex with the cationic surfactant cetylpiridinium chloride (CPC), quenching fluorescence. Upon addition of surfactin or other lipopeptides, fluorescein is released from the CPC-FL complex and quantified. The robustness of this method is assessed by comparing the quantification results to those conventionally measured by RP-UPLC and the results of strain screening are confirmed by MALDI-ToF analysis. The authors report for the first time the successful application of this analytical method for high-throughput screening of novel lipopeptide-producing strains. (AU)

FAPESP's process: 13/20570-6 - Sustainable production of biosurfactants with applications for medicine and environmental remediation
Grantee:Augusto Etchegaray Júnior
Support Opportunities: Regular Research Grants
FAPESP's process: 17/13330-0 - 10th World Congress of Chemical Engineering - WCCE10
Grantee:Augusto Etchegaray Júnior
Support Opportunities: Research Grants - Meeting - Abroad