Anti-infective agents are crucial drugs for life expectancy. Several reasons justify the need for new antibacterials, including high bacterial resistance and high mortality caused by infectious diseases. Thus, efforts are need to develop innovative antibacterial drugs. Recently, curcumin and cinnmaldehyde exhibited potent antibacterial activity. The central interest by these natural products is related to their bacterial cell division inhibitory effect, and their interaction with FtsZ protein (Filamentous temperature-sensitive protein Z), which is a vital macromolecule for all bacteria. The current project purposes the synthesis of three series of curcumin-cinnamaldehyde hybrids, which are methoxychalcones and their derivatives. These compounds will be prepared by aldol condensation reactions between ketone derivatives and aldehydes, under basic catalysis, as well as nucleophilic addition of alpha,beta-unsaturated ketones. Compounds will be tested against Gram-positive, Gram-negative bacteria and Mycobacterium species, using sensitive and resistant strains. The toxicity of selected antibacterial compounds will be evaluated on macrophages. The selective index (SI) of compounds will be calculated from IC50 and MBC values, IS = IC50/MBC. IC50 is a half maximal inhibitory concentration to kill macrophages and MBC is a minimum bactericidal concentration. Compounds displayed IS e 10 will be submitted to inhibitory bacterial cell division assays, using FtsZ sedimentation and GTP hydrolysis methods.
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