| Grant number: | 12/25237-0 |
| Support Opportunities: | Regular Research Grants |
| Start date: | April 01, 2013 |
| End date: | March 31, 2015 |
| Field of knowledge: | Biological Sciences - Microbiology - Applied Microbiology |
| Principal Investigator: | Carlos Henrique Gomes Martins |
| Grantee: | Carlos Henrique Gomes Martins |
| Host Institution: | Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil |
| City of the host institution: | Franca |
| Associated researchers: | Denise Crispim Tavares ; Lizandra Guidi Magalhães ; Sergio Ricardo Ambrosio |
Abstract
Among the diseases affecting the oral cavity, endodontic infections cause concern: microorganisms develop resistance, which is often a consequence of inefficient chemical-mechanical treatment. Endodontic infections have a polymicrobial nature; their treatment requires high concentrations of antimicrobial agents or even a combination of these agents, which can harm cells. Plant-derived compounds may be an important source of biologically active substances to treat bacterial diseases. This study aimed to (i) evaluate the antibacterial activity of kaurane-type diterpenes against the causative agents of endodontic infections, (ii) identify the proteins distinctly expressed in bacterial cells before and after action of the diterpene; and (iii) assess the cytotoxicity of the promising compounds. To determine the antibacterial potential of the tested compounds and calculate the Minimum Inhibitory Concentration (MIC), we will use the broth dilution method. We will obtain the Minimum Bactericidal Concentration (MBC), antibiofilm activity, bactericidal kinetics, and synergistic effect with chlorhexidine (Fractionated Inhibitory Concentration) for the metabolites with MIC d 10 ¼g.mL-1. We will conduct XTT assays, to investigate the cytotoxic activity. After incubation of the microorganisms with the promising compounds, analysis of global protein expression by proteomics will help identify the possible action mechanisms of the natural compounds. Hopefully, this project will pave the way for the discovery of chemical compounds potentially applicable as a complementary treatment in endodontics. (AU)
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