Abstract
Among the diseases affecting the oral cavity, endodontic infections cause concern: microorganisms develop resistance, which is often a consequence of inefficient chemical-mechanical treatment. Endodontic infections have a polymicrobial nature; their treatment requires high concentrations of antimicrobial agents or even a combination of these agents, which can harm cells. Plant-derived compounds may be an important source of biologically active substances to treat bacterial diseases. This study aimed to (i) evaluate the antibacterial activity of kaurane-type diterpenes against the causative agents of endodontic infections, (ii) identify the proteins distinctly expressed in bacterial cells before and after action of the diterpene; and (iii) assess the cytotoxicity of the promising compounds. To determine the antibacterial potential of the tested compounds and calculate the Minimum Inhibitory Concentration (MIC), we will use the broth dilution method. We will obtain the Minimum Bactericidal Concentration (MBC), antibiofilm activity, bactericidal kinetics, and synergistic effect with chlorhexidine (Fractionated Inhibitory Concentration) for the metabolites with MIC d 10 ¼g.mL-1. We will conduct XTT assays, to investigate the cytotoxic activity. After incubation of the microorganisms with the promising compounds, analysis of global protein expression by proteomics will help identify the possible action mechanisms of the natural compounds. Hopefully, this project will pave the way for the discovery of chemical compounds potentially applicable as a complementary treatment in endodontics. (AU)
Scientific publications
(11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES, JESSICA APARECIDA;
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DOS SANTOS MORAES, MARCOS FERNANDO;
SOLA VENEZIANI, RODRIGO CASSIO;
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ROCHA, BEATRIZ PATTI;
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Investigation of Safety Profile of Four Copaifera Species and of Kaurenoic Acid by Salmonella/Microsome Test.
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DA SILVA, DANIELLE FERNANDES;
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In vitro evaluation of essential oils for potential antibacterial effects against Xylella fastidiosa.
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Kaurenoic acid and its sodium salt derivative: antibacterial activity against Porphyromonas gingivalis and their mechanism of action.
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AMBROSIO, SERGIO RICARDO;
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ent-Copalic acid antibacterial and anti-biofilm properties against Actinomyces naeslundii and Peptostreptococcus anaerobius.
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BASTOS, JAIRO K.;
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Antibacterial Effect of Copaifera duckei Dwyer Oleoresin and Its Main Diterpenes against Oral Pathogens and Their Cytotoxic Effect.
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SOLA VENEZIANI, RODRIGO CASSIO;
AMBROSIO, SERGIO RICARDO;
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In vitro studies of the antibacterial activity of Copaifera spp. oleoresins, sodium hypochlorite, and peracetic acid against clinical and environmental isolates recovered from a hemodialysis unit.
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FIGUEIREDO ALMEIDA GOMES, BRENDA PAULA;
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Mikania glomerata Sprengel extract and its major compound ent-kaurenoic acid display activity against bacteria present in endodontic infections.
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SENEDESE, JULIANA MARQUES;
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VARANDA, ELIANA APARECIDA;
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Assessment of the antibacterial, cytotoxic and mutagenic potential of the phenolic-rich hydroalcoholic extract from Copaifera trapezifolia Hayne leaves.
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RODRIGUEZ BARDAJI, DANAE KALA;
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BIANCHI, THAMIRES CHIQUINI;
EUGENIO, DANIELE DE SOUZA;
DE OLIVEIRA, POLLYANNA FRANCIELLI;
LEANDRO, LUIS FERNANDO;
GHISLAIN ROGEZ, HERVE LOUIS;
SOLA VENEZIANNI, RODRIGO CASSIO;
AMBROSIO, SERGIO RICARDO;
TAVARES, DENISE CRISPIM;
BASTOS, JAIRO KENUPP;
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Copaifera reticulata oleoresin: Chemical characterization and antibacterial properties against oral pathogens.
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SOUZA, ARIANA B.;
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TAVARES, DENISE C.;
VENEZIANI, RODRIGO C. S.;
AMBROSIO, SERGIO R.;
BASTOS, JAIRO K.;
MARTINS, CARLOS H. G.
In vitro Evaluation of Copaifera oblongifolia Oleoresin Against Bacteria Causing Oral Infections and Assessment of Its Cytotoxic Potential.
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