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Kaurane-type diterpenes: activity against anaerobic bacteria and proteomics

Abstract

Among the diseases affecting the oral cavity, endodontic infections cause concern: microorganisms develop resistance, which is often a consequence of inefficient chemical-mechanical treatment. Endodontic infections have a polymicrobial nature; their treatment requires high concentrations of antimicrobial agents or even a combination of these agents, which can harm cells. Plant-derived compounds may be an important source of biologically active substances to treat bacterial diseases. This study aimed to (i) evaluate the antibacterial activity of kaurane-type diterpenes against the causative agents of endodontic infections, (ii) identify the proteins distinctly expressed in bacterial cells before and after action of the diterpene; and (iii) assess the cytotoxicity of the promising compounds. To determine the antibacterial potential of the tested compounds and calculate the Minimum Inhibitory Concentration (MIC), we will use the broth dilution method. We will obtain the Minimum Bactericidal Concentration (MBC), antibiofilm activity, bactericidal kinetics, and synergistic effect with chlorhexidine (Fractionated Inhibitory Concentration) for the metabolites with MIC d 10 ¼g.mL-1. We will conduct XTT assays, to investigate the cytotoxic activity. After incubation of the microorganisms with the promising compounds, analysis of global protein expression by proteomics will help identify the possible action mechanisms of the natural compounds. Hopefully, this project will pave the way for the discovery of chemical compounds potentially applicable as a complementary treatment in endodontics. (AU)

Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES, JESSICA APARECIDA; ABRAO, FARIZA; MORAES, THAIS DA SILVA; DAMASCENO, JAQUELINE LOPES; DOS SANTOS MORAES, MARCOS FERNANDO; SOLA VENEZIANI, RODRIGO CASSIO; AMBROSIO, SERGIO RICARDO; BASTOS, JAIRO KENUPP; DANTAS MIRANDA, MAYKER LAZARO; GOMES MARTINS, CARLOS HENRIQUE. Investigation of Copaifera genus as a new source of antimycobaterial agents. FUTURE SCIENCE OA, v. 6, n. 7 AUG 2020. Web of Science Citations: 0.
DAMASCENO, JAQUELINE LOPES; ARNET, YADIRA FERNANDEZ; FORTUNATO, GIOVANNA CAPALDI; GIROTTO, LUIZA; MARENA, GABRIEL DAVI; ROCHA, BEATRIZ PATTI; RESENDE, FLAVIA APARECIDA; AMBROSIO, SERGIO RICARDO; SOLA VENEZIANI, RODRIGO CASSIO; BASTOS, JAIRO KENUPP; GOMES MARTINS, CARLOS HENRIQUE. Investigation of Safety Profile of Four Copaifera Species and of Kaurenoic Acid by Salmonella/Microsome Test. Evidence-based Complementary and Alternative Medicine, 2019. Web of Science Citations: 1.
SANTIAGO, MARIANA BRENTINI; MORAES, THAIS DA SILVA; MASSUCO, JAQUELINE ESTETELE; SILVA, LARISSA OLIVEIRA; LUCARINI, RODRIGO; DA SILVA, DANIELLE FERNANDES; VIEIRA, TATIANA MANZINI; MILLER CROTTI, ANTONIO EDUARDO; GOMES MARTINS, CARLOS HENRIQUE. In vitro evaluation of essential oils for potential antibacterial effects against Xylella fastidiosa. JOURNAL OF PHYTOPATHOLOGY, v. 166, n. 11-12, p. 790-798, DEC 2018. Web of Science Citations: 1.
MARTINS, CARLOS H. G.; ABRAO, FARIZA; MORAES, THAIS S.; OLIVEIRA, POLLYANNA F.; TAVARES, DENISE C.; MAGALHAES, LIZANDRA G.; GALVAO, FABIO C.; VENEZIANI, RODRIGO C. S.; AMBROSIO, SERGIO R. Kaurenoic acid and its sodium salt derivative: antibacterial activity against Porphyromonas gingivalis and their mechanism of action. FUTURE MICROBIOLOGY, v. 13, n. 14, p. 1585-1602, NOV 2018. Web of Science Citations: 0.
MENDES DE SOUZA, MARIA GORETE; LEANDRO, LUIS FERNANDO; MORAES, THAIS DA SILVA; ABRAO, FARIZA; SOLA VENEZIANI, RODRIGO CASSIO; AMBROSIO, SERGIO RICARDO; GOMES MARTINS, CARLOS HENRIQUE. ent-Copalic acid antibacterial and anti-biofilm properties against Actinomyces naeslundii and Peptostreptococcus anaerobius. Anaerobe, v. 52, p. 43-49, AUG 2018. Web of Science Citations: 2.
ABRAO, FARIZA; ALVES, JESSICA A.; ANDRADE, GESSICA; DE OLIVEIRA, POLLYANNA F.; AMBROSIO, SERGIO R.; VENEZIANI, RODRIGO C. S.; TAVARES, DENISE C.; BASTOS, JAIRO K.; MARTINS, CARLOS H. G. Antibacterial Effect of Copaifera duckei Dwyer Oleoresin and Its Main Diterpenes against Oral Pathogens and Their Cytotoxic Effect. FRONTIERS IN MICROBIOLOGY, v. 9, FEB 21 2018. Web of Science Citations: 1.
LEITE VIEIRA, ROSIMARA GONCALVES; MORAES, THAIS DA SILVA; SILVA, LARISSA DE OLIVEIRA; BIANCHI, THAMIRES CHIQUINI; SOLA VENEZIANI, RODRIGO CASSIO; AMBROSIO, SERGIO RICARDO; BASTOS, JAIRO KENUPP; PIRES, REGINA HELENA; GOMES MARTINS, CARLOS HENRIQUE. In vitro studies of the antibacterial activity of Copaifera spp. oleoresins, sodium hypochlorite, and peracetic acid against clinical and environmental isolates recovered from a hemodialysis unit. ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, v. 7, JAN 24 2018. Web of Science Citations: 3.
CARRARA MORETI, DORA LUCIA; LEANDRO, LUIS FERNANDO; MORAES, THAIS DA SILVA; MOREIRA, MONIQUE RODRIGUES; SOLA VENEZIANI, RODRIGO CASSIO; AMBROSIO, SERGIO RICARDO; FIGUEIREDO ALMEIDA GOMES, BRENDA PAULA; GOMES MARTINS, CARLOS HENRIQUE. Mikania glomerata Sprengel extract and its major compound ent-kaurenoic acid display activity against bacteria present in endodontic infections. Anaerobe, v. 47, p. 201-208, OCT 2017. Web of Science Citations: 5.
LEANDRO, LUIS FERNANDO; MORAES, THAIS DA SILVA; DE OLIVEIRA, POLLYANNA FRANCIELLI; ALVES, JACQUELINE MORAIS; SENEDESE, JULIANA MARQUES; OZELIN, SAULO DUARTE; RESENDE, FLAVIA APARECIDA; DE GRANDIS, RONE APARECIDO; VARANDA, ELIANA APARECIDA; BASTOS, JAIRO KENUPP; TAVARES, DENISE CRISPIM; GOMES MARTINS, CARLOS HENRIQUE. Assessment of the antibacterial, cytotoxic and mutagenic potential of the phenolic-rich hydroalcoholic extract from Copaifera trapezifolia Hayne leaves. Journal of Medical Microbiology, v. 65, n. 9, p. 937-950, SEP 2016. Web of Science Citations: 7.
RODRIGUEZ BARDAJI, DANAE KALA; MANGABEIRA DA SILVA, JONAS JOAQUIM; BIANCHI, THAMIRES CHIQUINI; EUGENIO, DANIELE DE SOUZA; DE OLIVEIRA, POLLYANNA FRANCIELLI; LEANDRO, LUIS FERNANDO; GHISLAIN ROGEZ, HERVE LOUIS; SOLA VENEZIANNI, RODRIGO CASSIO; AMBROSIO, SERGIO RICARDO; TAVARES, DENISE CRISPIM; BASTOS, JAIRO KENUPP; MARTINS, CARLOS HENRIQUE G. Copaifera reticulata oleoresin: Chemical characterization and antibacterial properties against oral pathogens. Anaerobe, v. 40, p. 18-27, AUG 2016. Web of Science Citations: 21.
MORAES, THAIS DA S.; LEANDRO, LUIS F.; SILVA, LARISSA DE O.; SANTIAGO, MARIANA B.; SOUZA, ARIANA B.; FURTADO, RICARDO A.; TAVARES, DENISE C.; VENEZIANI, RODRIGO C. S.; AMBROSIO, SERGIO R.; BASTOS, JAIRO K.; MARTINS, CARLOS H. G. In vitro Evaluation of Copaifera oblongifolia Oleoresin Against Bacteria Causing Oral Infections and Assessment of Its Cytotoxic Potential. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, v. 17, n. 10, p. 894-904, 2016. Web of Science Citations: 7.

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