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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stability-indicating capillary zone electrophoresis assay for the analysis of linezolid in tablets

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Author(s):
Capistrano Goncalves de Oliveira, Cristiani Lopes [1] ; Nunes Salgado, Herida Regina [2] ; Leite Moraes, Maria de Lourdes [3]
Total Authors: 3
Affiliation:
[1] Univ Fed Ceara, Coll Pharm Dent & Nursing, Dept Pharm, Fortaleza, CE - Brazil
[2] State Univ Sao Paulo, Fac Pharmaceut Sci, Dept Drugs & Med, Araraquara, SP - Brazil
[3] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Chem, Diadema, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 54, n. 4 2018.
Web of Science Citations: 0
Abstract

A simple and fast alternative methodology using capillary zone electrophoresis (CZE) to analyze linezolid and its cationic photodegradation products in tablets has been developed. The separation was carried out on fused silica capillary and conducted using 100 mM formic acid (pH 3.0) and by applying 30 KV voltage. Detection was performed at UV 254 nm. The optimized method was validated in terms of linearity, limits of detection and quantification, precision (repeatability), stability studies (selectivity) and accuracy. Good linearity (8-20 mg L-1) was obtained and the limit of detection was 0.95 mg L-1. The greatest advantages of the CZE method were the rapid set-up of instrumentation and capillary equilibration, short analysis time (12 min), low running cost and low waste generation. The method showed good stability in determining linezolid submitted to degradation by light and to a climatic chamber and can be used as an alternative for evaluation in stability studies of linezolid in tablets, as well as for the analysis of the drug in raw materials and finished products. (AU)