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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae)

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Author(s):
Marina Cabral Ricoldi [1] ; Camila Soares Figueiredo [2] ; Janete Apparecida Desidério [3]
Total Authors: 3
Affiliation:
[1] Universidade Estadual Paulista “Júlio de Mesquita Filho” - Brasil
[2] Universidade Estadual Paulista “Júlio de Mesquita Filho” - Brasil
[3] Universidade Estadual Paulista “Júlio de Mesquita Filho” - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Arq. Inst. Biol.; v. 85, 2018-11-23.
Abstract

ABSTRACT: Bacillus thuringiensis subsp. israelensis has been used to control the Aedes aegypti (Diptera: Culicidae) mosquito larvae, the vector of virus diseases such as dengue, Chikungunya and Zika fever, which have become a major public health problem in Brazil and other tropical countries since the climate favors the proliferation and development of the transmitting vector. Because B. thuringiensis has shown potential for controlling insects of the Diptera order, this work aimed at testing the Bacillus thuringiensis subsp. thuringiensis strain T01-328 and its proteins Cry2Aa and Cry2Ab for control A. aegypti and at comparing the results to the B. thuringiensis subsp. israelensis specific dipteran strain. To this end, bioassays using spore-crystal of both strains, and Cry2Aa and Cry2Ab proteins from the heterologous expression in Escherichia coli, were performed against A. aegypti larvae. The results showed that the B. thuringiensis thuringiensis ­T01-328 has insecticidal activity against the larvae, but it is less toxic than B. thuringiensis subsp. israelensis. Cry2Aa and Cry2Ab proteins expressed heterologously were effective for controlling A. aegypti larvae. Therefore, the results indicate that the Cry2Aa and Cry2Ab proteins of the B. thuringiensis thuringiensis T01-328 can be used as an alternative to assist in the control of A. aegypti. (AU)

FAPESP's process: 16/03108-5 - Expression of genes Cry2Aa and Cry2Ab of Bacillus thuringiensis T01 328 and evaluation of toxicity to larvae of Aedes aegypti (Diptera: Culicidae)
Grantee:Marina Cabral Ricoldi
Support Opportunities: Scholarships in Brazil - Scientific Initiation