| Full text | |
| Author(s): |
Frances Lilian Lanhellas Gonçalves
[1]
;
Fábio Santana de Oliveira
[2]
;
Augusto Frederico Schmidt
[3]
;
Luís Antônio Violin Dias Pereira
[4]
;
Rodrigo Melo Gallindo
[5]
;
Rebeca Lopes Figueira
[6]
;
Lourenço Sbragia
[7]
Total Authors: 7
|
| Affiliation: | [1] University of Sao Paulo. School of Medicine of Ribeirao Preto. Department of Surgery and Anatomy - Brasil
[2] University of Sao Paulo. School of Medicine - Brasil
[3] Cincinnati Children's Hospital Medical Center. Department of Pediatrics - Estados Unidos
[4] State University of Campinas. Department of Histology and Embryology - Brasil
[5] University of Sao Paulo. School of Medicine of Ribeirao Preto. Department of Surgery and Anatomy - Brasil
[6] University of Sao Paulo. School of Medicine of Ribeirao Preto. Department of Surgery and Anatomy - Brasil
[7] University of Sao Paulo. School of Medicine of Ribeirao Preto. Department of Surgery and Anatomy - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Acta Cirurgica Brasileira; v. 28, p. 13-18, 2013-00-00. |
| Abstract | |
PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin.} RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p <0.001). HA was decreased on GD 18.5 compared to 21.5 (p <0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased. (AU) | |
| FAPESP's process: | 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen |
| Grantee: | Lourenço Sbragia Neto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats |
| Grantee: | Frances Lilian Lanhellas Gonçalves |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |