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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Myxosporidiosis in intensively-reared Piaractus mesopotamicus: Histopathological diagnosis by means of Ziehl-Neelsen staining

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Author(s):
Wilson G. Manrique [1] ; Gustavo S. Claudiano [2] ; Mayra A.P. Figueiredo [3] ; Thalita R. Petrillo [4] ; Julieta R.E. Moraes [5] ; Flávio R. de Moraes [6]
Total Authors: 6
Affiliation:
[1] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[2] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[3] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[4] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[5] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[6] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 32, n. 11, p. 1133-1137, 2012-11-00.
Abstract

Samples of different organs from intensively-reared Piaractus mesopotamicus were collected and processed using routine histological techniques in order to produce thin sections for staining with hematoxylin-eosin and with the Ziehl-Neelsen method. Through examination under an optical microscope, myxosporidians of the genera Henneguya sp. and Myxobolus sp. were identified, respectivelyin the gills and kidneys of P. mesopotamicus. Plasmodia with immature spores of Henneguya sp. were located along the secondary lamellae, with total length of 30.45±4.84µm and width of 3.52±0.33µm. Spores of Myxobolus sp. were located in the kidneys, with total length of 8.94±0.82µm and width of 5.59±0.39µm. Histopathological analysis of the gills showed plasmodia containing spores of Henneguya sp., at intralamellar and intravascular localities, at different stages of development. Spores of Myxobolus sp. were identified in the kidneys, in the peritubular region and in the interstices and glomerulus, surrounded by melanomacrophages. Focal hemorrhage was recorded in a few cases. Ziehl-Neelsen staining allowed to identify particular features of the spores and facilitated biometry and enabled classification in comparison with hematoxylin-eosin, thus demonstrating its usefulness for histopathological diagnosis of the parasitosis. (AU)

FAPESP's process: 09/17640-7 - COMPARATIVE STUDY OF INDUCED CHRONIC INFLAMMATORY RESPONSE IN PACU (Piaractus mesopotamicus) WITH FOREIGN BODY AND BCG
Grantee:Wilson Gómez Manrique
Support Opportunities: Scholarships in Brazil - Doctorate