Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL 18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

Author(s)

André Roberto Bussmann ^[1] ; Marcos Antônio Marton Filho ^[2] ; Marília Pinheiro Módolo ^[3] ; Renata Pinheiro Módolo ^[4] ; Patrícia Amado ^[5] ; Maria Aparecida Custódio Domingues ^[6] ; Yara Marcondes Machado Castiglia ^[7] ; Norma Sueli Pinheiro Módolo ^[8]

Affiliation

^[1] Sao Paulo State University. Botucatu Medical School - Brasil

^[2] UNESP. Botucatu Medical School - Brasil

^[3] UNESP. Botucatu Medical School - Brasil

^[4] ABC Medical School - Brasil

^[5] UNESP. Botucatu Medical School - Brasil

^[6] UNESP. Botucatu Medical School. Department of Pathology - Brasil

^[7] UNESP. Botucatu Medical School. Department of Anaesthesiology - Brasil

^[8] UNESP. Botucatu Medical School. Department of Anaesthesiology - Brasil

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 29, n. 8, p. 515-521, 2014-08-00.

Abstract

PURPOSE:To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM 1 and IL 18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury.METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg·kg1·d 1; and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg·kg 1·d1. The NGALp, NGALu, KIM 1, IL 18 and creatinine levels and the kidney histology were analysed. The significance level was established as p<0.05.RESULTS:Creatinine level increased in all the groups, with A ≈ C > S ≈ CA. The NGALp, NGALu and IL 18 levels exhibited similar behaviour in all the groups. KIM 1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA.CONCLUSION:Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury. (AU)

• FAPESP's process: 12/13604-9 - Effects of allopurinol in renal protection by measurement of plasma NGAL: a study in an experimental model of ischemia and reperfusion in rats under ether anesthesia
• Grantee: Norma Sueli Pinheiro Módolo
• Support Opportunities: Regular Research Grants