Antimicrobial Activity of Compounds Containing Silver Nanoparticles and Calcium Glycerophosphate in Combination with Tyrosol

Author(s):Show less -

Santos Souza, Jose Antonio ^[1] ; Barbosa, Debora Barros ^[1] ; do Amaral, Jackeline Gallo ^[1] ; Monteiro, Douglas Roberto ^[2] ; Gorup, Luiz Fernando ^[3] ; de Souza Neto, Francisco Nunes ^[4] ; Fernandes, Renan Aparecido ^[5] ; Fernandes, Gabriela Lopes ^[1] ; de Camargo, Emerson Rodrigues ^[4] ; Agostinho, Alessandra Marcal ^[6] ; Botazzo Delbem, Alberto Carlos ^[1]

Affiliation

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Univ Western Sao Paulo UNOESTE, GPD Masters Degree, Presidente Prudente, SP - Brazil

^[3] Fed Univ Grande Dourados, Dourados, MS - Brazil

^[4] Univ Fed Sao Carlos, Sao Carlos, SP - Brazil

^[5] Univ Ctr Adamantina UNIFAI, Adamantina, SP - Brazil

^[6] Michigan State Univ, E Lansing, MI 48824 - USA

• Document type: Journal article
• Source: INDIAN JOURNAL OF MICROBIOLOGY; v. 59, n. 2, p. 147-153, JUN 2019.
• Web of Science Citations: 0

Abstract

Nanocomposites containing antimicrobial agents and calcium phosphates have been developed. Thus, this study assessed the effects of two compounds containing silver nanoparticles (AgNPs) and -calcium glycerophosphate (CaGP), associated or not with tyrosol (TYR), against planktonic cells and biofilms of Candida albicans and Streptococcus mutans. The nanocompounds were synthesized through chemical and green' processes and characterized by scanning electron microscopy. The minimum and fractional inhibitory concentrations of each compound were determined for planktonic cells. Next, 24-h single biofilms of C. albicans and S. mutans were treated for 24h with the nanocompounds alone or in combination with TYR, and the antibiofilm effect was assessed through enumeration of colony forming units. Biofilm data were statistically examined using one-way ANOVA and the Kruskal-Wallis test (=0.05). The chemically synthesized nanocompound in combination with TYR demonstrated a synergistic effect against planktonic cells of C. albicans and S. mutans. For the nanocompound obtained through the green' route associated with TYR, a synergistic effect was observed only against C. albicans. For biofilms, only the combination obtained through the green' route+TYR demonstrated a synergistic effect against C. albicans. Our results may contribute to the development of oral care products containing AgNPs-CaGP and TYR to combat oral infections. (AU)

• FAPESP's process: 15/00825-5 - Effect of the calcium glycerophosphate associated with nanoparticles of colloidal silver obtained by the 'green' synthesis about biofilms of Candida albicans and Streptococcus mutans
• Grantee: José Antonio Santos Souza
• Support Opportunities: Scholarships in Brazil - Doctorate