| Full text | |
| Author(s): |
de Araujo Baptista, Vivianne Izabelle
[1]
;
Quintana, Hananiah Tardivo
[1]
;
Lazzarin, Mariana Cruz
[1]
;
Benfato, Izabelle Dias
[1]
;
De Carvalho, Francine Pereira
[1]
;
Le Sueur-Maluf, Luciana
[1]
;
Machado De Oliveira, Camila Aparecida
[1]
;
Baptista, Josemberg da Silva
[2]
;
De Oliveira, Flavia
[1]
Total Authors: 9
|
| Affiliation: | [1] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim 136 Lab 328, BR-11015020 Santos, SP - Brazil
[2] Fed Univ Espirito Santo UFES, Dept Morphol, Vitoria, ES - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Connective Tissue Research; v. 60, n. 3, p. 230-239, MAY 4 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Extensive burn may cause acute resistance to insulin, which accentuates hypermetabolism, impairs glucose metabolism, immune dysfunction and risks of sepsis. To minimize these effects, insulin is used as a treatment. The purpose was to analyze the collagen-elastic arrangement effects of insulin on the burned skin. Wistar rats were assigned in groups: control (C); control with insulin (C + I); scald burn injury (SBI); and SBI with insulin (SBI+ I). SBI were submitted to 45% total body surface area burn and the insulin-treated groups received insulin (5 UI/Kg/day) for 4 or 14 days (d). Insulin levels, glucose tolerance test and HOMA index were determined. The skin sections were analyzed for histophatological and morphoquantitative data. Histopathological findings showed increased reepithelization of SBI+ I and formation of a new muscle layer after 14 days. In the collagen-elastic arrangement, insulin for 4 days increased the volume fraction (Vv) of thin collagen and elastic fibers. After 14 days, independently of injury, insulin decreased the elastic fibers. Insulin was able to reverse damages in the collagen-elastic rearrangement and stimulate reepithelization after 4 days. Untreated scald-burned animals showed higher Vv of thick collagen after 4 days, while those treated had a higher Vv of thin collagen. The Vv of elastic fibers was increased in SBI+ I for 4 days. In conclusion, insulin treatment was able to stimulate reepithelization. It also reversed the damages to the collagen-elastic arrangement in the scald-burned group, improving the organization of thin collagen and increasing the Vv of elastic fibers in the injured group treated with insulin for a short time, that is, for 4 days. (AU) | |
| FAPESP's process: | 14/11458-0 - Morphoquantitative and molecular aspects of skeletal muscle of rats submitted to treatment with insulin after burn injury |
| Grantee: | Hananiah Tardivo Quintana |
| Support Opportunities: | Scholarships in Brazil - Doctorate |