| Full text | |
| Author(s): |
Ramiro, Juliana
[1]
;
Ciampi-Guillardi, Maisa
[1]
;
Gomes Caldas, Danielle Gregorio
[2]
;
Duarte de Moraes, Maria Heloisa
[1]
;
Goldoni Barbieri, Marina Coan
[1]
;
Pereira, Wagner Vicente
[3]
;
Massola, Jr., Nelson Sidnei
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Dept Plant Pathol & Nemathol, ESALQ, Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, SP - Brazil
[3] Univ Fed Parana, Dept Crop Sci, Curitiba, PR - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF PHYTOPATHOLOGY; v. 167, n. 5, p. 273-282, MAY 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Seed-borne pathogenic fungi can cause serious damage to soybean crops by reducing the germination, vigour and emergence of the seeds. Special attention should be paid to pathogen detection in seeds to prevent its introduction in disease-free areas. Considering the importance of rapid and successful diagnosis of seed-borne pathogenic fungi in soybeans, this study evaluated a method to detect Sclerotinia sclerotiorum and Phomopsis spp. in seeds using quantitative polymerase chain reaction (qPCR). Naturally infested samples were subjected to detection using qPCR and blotter test, and the findings were compared. Using soybean seeds soaked in water, both pathogens were detected at an infestation level up a 0.0625% (one infected seed out of 1,599 healthy seeds) by qPCR. This technique allowed the detection of 300 fg of S. sclerotiorum and 30 fg of Phomopsis spp. DNA in the seed samples. Phomopsis spp. was detected in 40.7% of the evaluated seed batches (81 batches) and S. sclerotiorum was detected in 32.1% of the evaluated batches, although most of the seeds had low infestation levels. It was up to 28.5 times more efficient to use qPCR rather than blotter test to detect pathogens with a low incidence of occurrence in soybean seeds. If routinely used to test healthy seeds, qPCR would contribute to reducing soybean losses due to diseases as well as decreasing the costs required to control those diseases. (AU) | |
| FAPESP's process: | 13/16726-0 - Molecular detection of seedborne pathogenic fungi in soybean |
| Grantee: | Nelson Sidnei Massola Júnior |
| Support Opportunities: | Regular Research Grants |