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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A preliminary investigation of circulating extracellular vesicles and biomarker discovery associated with treatment response in head and neck squamous cell carcinoma

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Author(s):
Rodrigues-Junior, Dorival Mendes [1, 2] ; Tan, Soon Sim [3] ; Viana, Luciano de Souza [4] ; Carvalho, Andre Lopes [4] ; Lim, Sai Kiang [3] ; Iyer, N. Gopalakrishna [1, 5] ; Vettore, Andre Luiz [2]
Total Authors: 7
Affiliation:
[1] Natl Canc Ctr Singapore, Canc Therapeut Res Lab, 11 Hosp Dr, Singapore 169610 - Singapore
[2] Univ Fed Sao Paulo, Dept Biol Sci, Lab Biol Mol Canc, UNIFESP, Rua Pedro de Toledo 669, 11 Andar, BR-04039032 Sao Paulo, SP - Brazil
[3] ASTAR, Inst Med Biol, Singapore - Singapore
[4] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil
[5] Natl Canc Ctr Singapore, Div Surg Oncol, Singapore - Singapore
Total Affiliations: 5
Document type: Journal article
Source: BMC CANCER; v. 19, APR 23 2019.
Web of Science Citations: 2
Abstract

BackgroundThere is a paucity of plasma-based biomarkers that prospectively segregate the outcome of patients with head and neck squamous-cell carcinoma (HNSCC) treated with chemoradiation therapy (CRT). Plasma extracellular vesicles (EVs) might be an alternative source for discovery of new specific markers present in patients with HNSCC, which could help to re-direct patients to appropriate curative therapies without delay.MethodsIn order to identify new markers in plasma compartments, Cholerae toxin B chain (CTB) and Annexin V (AV) were used to isolate EVs from pooled plasma samples from patients with locally advanced HNSCC who responded (CR, n=6) or presented incomplete response (NR, n=6) to CRT. The crude plasma and EVs cargo were screened by antibody array.ResultsOf the 370 polypeptides detected, 119 proteins were specific to NR patients while 38 were exclusive of the CR subjects. The Gene Set Enrichment Analysis (GSEA) and Search Tool for the Retrieval of Interacting Genes (STRING) database analysis indicated that the content of circulating plasma EVs might have a relevant function for the tumor intercellular communication in the HNSCC patients.ConclusionThis study provides a list of potential markers present in plasma compartments that might contribute to the development of tools for prediction and assessment of CRT response and potentially guide therapeutic decisions in this context. (AU)

FAPESP's process: 15/21420-3 - Analysis content of the extracellular vesicles present in the plasma of head and neck squamous cell carcinoma patients for identification of molecular markers to treatment response
Grantee:Dorival Mendes Rodrigues Junior
Support type: Scholarships in Brazil - Doctorate