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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of glucose concentration on productivity and yield of hydrogen production by the new isolate Clostridium beijerinckii Br21

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Author(s):
Fonseca, Bruna Constante [1] ; Schmidell, Willibaldo [2] ; Reginatto, Valeria [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Lab Biotecnol Ambiental & Energias Renovaveis, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Fed Santa Catarina, Dept Engn Quim & Engn Alimentos, Florianopolis, SC - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CANADIAN JOURNAL OF CHEMICAL ENGINEERING; v. 97, n. 5, p. 1092-1099, MAY 2019.
Web of Science Citations: 1
Abstract

This paper presents a detailed analysis of the batch kinetic fermentative assays data used to evaluate how glucose concentration influences fermentative H-2 production, including the non-dissociated acid concentration threshold, with the new isolate Clostridium beijerinckii Br21. Fermentative batch assays for hydrogen (H-2) production were conducted with initial glucose concentrations of 5.2, 27.2, 54.4, 97.2, or 154.4mmol/L without pH control. The dry cell mass (X), glucose (S), and product (H-2) concentrations and the pH were monitored for 60h. Increasing initial glucose concentration raised the maximum specific H-2 production rate to values as high as 1.19mmol H-2/mg X<bold>h</bold>. However, the H-2 production yield (Y-H2/S) decreased from 2.23 to 0.2mmol H-2/mmol glucose. The initial glucose concentration that provided the best compromise between Y-H2/S and H-2 productivity was 37mmol/L (6.7g/L). The lactic acid, acetic acid, and butyric acid concentrations enhanced at higher initial glucose concentrations, leading to the largest pH decrease. Lower pH favoured non-dissociated acids, which switched the Clostridium metabolism to solventogenesis. The threshold acid concentrations for C. beijerinckii Br21 solventogenesis were non-dissociated butyric acid at 8mmol/L and total non-dissociated acetic and butyric acids at 13mmol/L. Glucose supply and pH control in continuous or fed-batch biorreactors could culminate in higher H-2 productivity and yield by C. beijerinckii Br21. (AU)

FAPESP's process: 15/06074-1 - Third-generation biohydrogen production from algae biomass and its hydrolysis derivatives
Grantee:Valeria Reginatto Spiller
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants