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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Manganese-induced cellular disturbance in the baker's yeast, Saccharomyces cerevisiae with putative implications in neuronal dysfunction

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Author(s):
Hernandez, Raul Bonne [1, 2, 3] ; Moteshareie, Houman [1, 2] ; Burnside, Daniel [1, 2] ; McKay, Bruce [1, 2] ; Golshani, Ashkan [1, 2]
Total Authors: 5
Affiliation:
[1] Carleton Univ, Dept Biol, 1125 Colonel By Dr, Ottawa, ON K1S 5B6 - Canada
[2] Carleton Univ, Ottawa Inst Syst Biol, 1125 Colonel By Dr, Ottawa, ON K1S 5B6 - Canada
[3] Univ Fed Sao Paulo, Dept Quim, Lab Bioinorgan & Toxicol Ambiental LABITA, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, APR 25 2019.
Web of Science Citations: 0
Abstract

Manganese (Mn) is an essential element, but in humans, chronic and/or acute exposure to this metal can lead to neurotoxicity and neurodegenerative disorders including Parkinsonism and Parkinson's Disease by unclear mechanisms. To better understand the effects that exposure to Mn2+ exert on eukaryotic cell biology, we exposed a non-essential deletion library of the yeast Saccharomyces cerevisiae to a sub-inhibitory concentration of Mn2+ followed by targeted functional analyses of the positive hits. This screen produced a set of 43 sensitive deletion mutants that were enriched for genes associated with protein biosynthesis. Our follow-up investigations demonstrated that Mn reduced total rRNA levels in a dose-dependent manner and decreased expression of a beta-galactosidase reporter gene. This was subsequently supported by analysis of ribosome profiles that suggested Mn-induced toxicity was associated with a reduction in formation of active ribosomes on the mRNAs. Altogether, these findings contribute to the current understanding of the mechanism of Mn-triggered cytotoxicity. Lastly, using the Comparative Toxicogenomic Database, we revealed that Mn shared certain similarities in toxicological mechanisms with neurodegenerative disorders including amyotrophic lateral sclerosis, Alzheimer's, Parkinson's and Huntington's diseases. (AU)

FAPESP's process: 16/00371-7 - Toxicogenomic cross-species studies to develop an adverse outcome pathway framework for understanding and predicting both neurotoxicity during development and manganese-induced neurodegeneration
Grantee:Raúl Bonne Hernández
Support type: Scholarships abroad - Research
FAPESP's process: 16/50483-6 - Modeling in yeast the neurotoxicity and neurodegeneration induced by manganese
Grantee:Raúl Bonne Hernández
Support type: Regular Research Grants