Performance of wastewater reclamation systems in enteric virus removal

Author(s)

Prado, Tatiana ^[1] ; Bruni, Antonio de Castro ^[1] ; Funada Barbosa, Mikaela Renata ^[1] ; Garcia, Suzi Cristina ^[1] ; de Jesus Melo, Adalgisa Maria ^[1] ; Zanoli Sato, Maria Ines ^[1]

Affiliation

^[1] Environm Co Sao Paulo State CETESB, Div Microbiol & Parasitol, Dept Environm Anal, Av Prof Frederico Hermann Jr 345, BR-05459900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Science of The Total Environment; v. 678, p. 33-42, AUG 15 2019.
• Web of Science Citations: 0

Abstract

Analysis of virus removal by tertiary or advanced sewage treatment processes is an emerging topic due to importance of reusing water on a global level. This study aimed to monitor important human viral pathogens: the human adenovirus (HAdV), JC polyomavirus (JCV) and Species A rotaviruses (RVA) in urban sewage, secondary effluents and reclaimed water from metropolitan Sao Paulo (MSP), Brazil. Four large wastewater treatment plants (WWTPs) in MSP were sampled monthly during a one-year period (April 2015 to March 2016). The viruses were quantified by quantitative PCR (qPCR), and HAdV viability was tested by the integrated cell culture (ICC)-qPCR assay. WWTPs are composed of activated sludge processes and different tertiary treatments (coagulation/sedimentation, sand-anthracite filters, membrane bioreactors (MBRs)/reverse osmosis (RO) and disinfection by chlorination). Physicochemical parameters were also evaluated to verify association with density of viruses detected indifferent treatment stages. HAdV, JCV and RVA were consistently detected (100%) in the sewage influent samples (range: 10(6)-10(8) genome copies GC/L). In the secondary effluent, HAdV was detected in 100% (48/48) of the analysed samples, JCV in 85.4% and RVA in 97.9% (range: 10(4)-10(7) GC/L for all viruses tested). HAdV was the most frequently detected virus in the tertiary effluent (62.2%) (28/45), exhibiting a viability between 0 and 44% of the tested samples in the wastewater reclamation systems. The MBR/RO systems demonstrated better virus removal efficiencies (range: 2.3-2.9 log(10)). Temperature, pH, turbidity and total organic carbon presented association with the viral density in the reclaimed water samples. Presence of viruses in treated effluents can indicates health risks depending on uses of recovery water. Further risk assessment studies should be conducted to better assess health risks under different exposure scenarios for water recovery in urban settings. (C) 2019 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/26586-1 - Emerging enteric viruses in wastewater from São Paulo city: Epidemiological surveillance
• Grantee: Tatiana Prado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral