A Fast and Simple Procedure for Polybrominated Diphenyl Ether Determination in Egg Samples by Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry

Author(s)

Oliveira Souza, Marilia Cristina ^[1] ; Rocha, Bruno Alves ^[1] ; Oliveira Souza, Juliana Maria ^[1] ; Berretta, Andresa Aparecida ^[2] ; Barbosa, Jr., Fernando ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, ASTox Lab Toxicol Analit & Sistemas, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Apis Flora Ind & Comercial Ltda, Lab Pesquisa Desenvolvimento & Inovacao, BR-14020670 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: FOOD ANALYTICAL METHODS; v. 12, n. 7, p. 1528-1535, JUL 2019.
• Web of Science Citations: 0

Abstract

Polybrominated diphenyl ethers (PBDEs) are a class of flame retardants used in numerous consumer products to increase product resistance to fire and/or high temperatures. However, several studies suggest potential harmful effects on human health after exposure to these compounds. Since diet is a relevant source of PBDE exposure, food samples must be routinely monitored to check for possible contamination. On the other hand, routine food monitoring procedures require fast, simple, sensitive, and selective analytical methodologies. Therefore, in this study, an original method combining microextraction by packed sorbent and gas chromatography coupled to mass spectrometry for the determination of six congeners of PBDEs (BDE-28, BDE-47, BDE-99, BDE-100, BDE-153, and BDE-154) in egg samples was proposed. The attractive advances over current protocols include the reduced sample amount required (50mg of freeze-dried egg), the low solvent volumes (lower than 300L), the lower detection limit (LOD=0.42 ng/glw), and an excellent reproducibility and repeatability (<10 and 8%, respectively). Furthermore, the method is fast and simple. After validation, the proposed method was applied to the determination of PBDEs in 40 samples, including free-range and conventional eggs commercialized in Brazil. The free-range eggs presented the highest detection percentage of PBDEs, corresponding to 67.7%, and BDE-47 was the predominant PBDE found (30% of samples). The mean value observed for BDE-47 was 5.9 ng/g lipid weight (lw), whereas the sum of all analyzed PBDEs (Sigma(PBDEs)) was 16.7 ng/glw. (AU)

• FAPESP's process: 15/19665-8 - Determination of the main representatives of polybrominated diphenyl ethers class (PBDEs) in fish, eggs and milk employing microextraction packed sorbent (MEPS) and gas chromatography coupled with mass spectrometry (GC-MS)
• Grantee: Marília Cristina Oliveira Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/02949-6 - The development of analytical procedures for Hg and as speciation in environmental and biological samples by HPLC-ICP-MS and sample preparation with microextraction by packed sorbent (MEPS)
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Regular Research Grants