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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new heparan sulfate from the mollusk Nodipecten nodosus inhibits merozoite invasion and disrupts rosetting and cytoadherence of Plasmodium falciparum

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Bastos, Marcele F. [1] ; Albrecht, Letusa [2] ; Gomes, Angelica M. [3] ; Lopes, Stefanie C. P. [4] ; Vicente, Cristina P. [5] ; de Almeida, Rodrigo P. M. [2] ; Cassiano, Gustavo C. [1] ; Fonseca, Roberto J. C. [6] ; Werneck, Claudio C. [7] ; Pavao, Mauro S. G. [8] ; Costa, Fabio T. M. [1]
Total Authors: 11
Affiliation:
[1] Univ Estadual Campinas, Dept Genet Evolucao Microbiol & Imunol, Lab Doencas Trop, Campinas, SP - Brazil
[2] Fundacao Oswaldo Cruz FIOCRUZ, Inst Carlos Chagas, Curitiba, Parana - Brazil
[3] Cleveland Clin, Lerner Res Inst, Dept Biomed Engn, Cleveland, OH 44106 - USA
[4] Fundacao Oswaldo Cruz FIOCRUZ, Inst Leonidas & Maria Deane, Manaus, Amazonas - Brazil
[5] Univ Estadual Campinas, Dept Biol Estrutural & Func, Campinas, SP - Brazil
[6] Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Ctr Ciencias Saude, Inst Ciencias Biomed, Lab Tecido Conjunt, Rio De Janeiro, RJ - Brazil
[7] Univ Estadual Campinas, Dept Bioquim & Biol Tecidual, Campinas, SP - Brazil
[8] Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Programa Glicobiol, Rio De Janeiro, RJ - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 114, 2019.
Web of Science Citations: 0
Abstract

BACKGROUND Despite treatment with effective antimalarial drugs, the mortality rate is still high in severe cases of the disease, highlighting the need to find adjunct therapies that can inhibit the adhesion of Plasmodium falciparum-infected erythrocytes (Pf-iEs). OBJECTIVES In this context, we evaluated a new heparan sulfate (HS) from Nodipecten nodosus for antimalarial activity and inhibition of P. falciparum cytoadhesion and rosetting. METHODS Parasite inhibition was measured by SYBR green using a cytometer. HS was assessed in rosetting and cytoadhesion assays under static and flow conditions using Chinese hamster ovary (CHO) and human lymphatic endothelial cell (HLEC) cells expressing intercellular adhesion molecule-1 (ICAM1) and chondroitin sulfate A (CSA), respectively. FINDINGS This HS inhibited merozoite invasion similar to heparin. Moreover, mollusk HS decreased cytoadherence of P. falciparum to CSA and ICAM-1 on the surface of endothelial cells under static and flow conditions. In addition, this glycan efficiently disrupted rosettes. CONCLUSIONS These findings support a potential use for mollusk HS as adjunct therapy for severe malaria. (AU)

FAPESP's process: 17/18611-7 - Development of new tools for search and validation of molecular targets for therapy against Plasmodium vivax
Grantee:Fabio Trindade Maranhão Costa
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/16525-2 - Plasmodium vivax: pathogenesis and infectivity
Grantee:Fabio Trindade Maranhão Costa
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 10/18571-6 - Fucosylated chondroitin sulfate and an heparin analog effect on Plasmodium falciparum cytoadhesion and merozoite invasion
Grantee:Marcele Fontenelle Bastos
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/20774-6 - Identifying protein kinase inhibitors as antimalarial agents using chemogenomic, bioinfomatics and phenotypic strategies: focus in Plasmodium vivax.
Grantee:Gustavo Capatti Cassiano
Support Opportunities: Scholarships in Brazil - Post-Doctoral