| Full text | |
| Author(s): |
dos Santos, Jeniffer Farias
[1, 2]
;
Borcari, Nathalia Ruder
[1]
;
Araujo, Mariana da Silva
[2]
;
Nunes, Viviane Abreu
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Cellular Biochemistry; v. 120, n. 8, p. 13141-13155, AUG 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Epidermal differentiation is a complex process in which keratinocytes go through morphological and biochemical changes in approximately 15 to 30 days. Abnormal keratinocyte differentiation is involved in the pathophysiology of several skin diseases. In this scenario, mesenchymal stem cells (MSCs) emerge as a promising approach to study skin biology in both normal and pathological conditions. Herein, we have studied the differentiation of MSC from umbilical cord into keratinocytes. MSC were cultured in Dulbecco's modified Eagle's medium (DMEM) (proliferation medium) and, after characterization, differentiation was induced by culturing cells in a defined keratinocyte serum-free medium (KSFM) supplemented with epidermal growth factor (EGF) and calcium chloride ions. Cells cultivated in DMEM were used as control. Cultures were evaluated from day 1 to 23, based on the cell morphology, the expression of p63, involucrin and cytokeratins (KRTs) KRT5, KRT10 and KRT14, by quantitative polymerase chain reaction, Western blot analysis or immunofluorescence, and by the detection of epidermal kallikreins activity. In cells grown in keratinocyte serum-free medium with EGF and 1.8 mM calcium, KRT5 and KRT14 expression was shown at the first day, followed by the expression of p63 at the seventh day. KRT10 expression was detected from day seventh while involucrin was observed after this period. Data showed higher kallikrein (KLK) activity in KSFM-cultured cells from day 11th in comparison to control. These data indicate that MSC differentiated into keratinocytes similarly to that occurs in the human epidermis. KLK activity detection appears to be a good methodology for the monitoring the differentiation of MSC into the keratinocyte lineage, providing useful tools for the better understanding of the skin biology. (AU) | |
| FAPESP's process: | 13/23245-9 - Differentiation of mesenchymal stem cells from umbilical cord into keratinocytes: study of biology of the epidermis and perspectives for understanding the development of diseases |
| Grantee: | Jeniffer Farias dos Santos |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/03503-3 - Establishment of a model for investigating the in vitro differentiation of mesenchymal stem cells from umbilical cord blood into keratinocytes: an approach to study the biology of the skin |
| Grantee: | Viviane Abreu Nunes Cerqueira Dantas |
| Support Opportunities: | Regular Research Grants |