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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Panax ginseng methabolit (GIM-1) prevents oxidative stress and apoptosis in human Sertoli cells exposed to Monobutyl-phthalate (MBP)

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Author(s):
De Freitas, Andre Teves A. G. [1] ; Pinho, Cristiane Figueiredo [1] ; de Aquino, Ariana Musa [1] ; Fernandes, Ana Angelica H. [2] ; Domeniconi, Raquel Fantin [3] ; Justulin, Luis Antonio [1] ; Scarano, Wellerson Rodrigo [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, BR-18618689 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618689 Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Anat, BR-18618689 Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: REPRODUCTIVE TOXICOLOGY; v. 86, p. 68-75, JUN 2019.
Web of Science Citations: 0
Abstract

This study evaluated oxidative stress markers in Human Sertoli cells cultivated on Geltrex (R) and exposed to Monobutyl Phthalate (MBP), and the potential cytoprotective role of GIM-1 on the antioxidant response. Exposure was performed at 30 min, 1, 12 and 48 h into 4 groups: control, MBP (10 mu M), GIM-1 (0,05 mu M) and MBP + GIM-1. Morphology was evaluated. Antioxidant enzymes were analyzed by colorimetric method; NRF-2, SIRT-1, 8- OHdG and Cleaved Caspase-3 by Western Blot. Larger spaces between cells were shown in MBP treatment; GIM-1 was similar to Control and MBP + GIM-1 showed an intermediate aspect. MBP reduced enzymatic activity of all enzymes and NRF-2 expression, increasing cleaved Caspase-3 expression; while GIM-1 increased antioxidants markers alone and attenuated MPB effects in MBP + GIM-1. MBP induced deleterious effects on Sertoli cells, increasing the oxidative stress, apoptosis and modifying their distribution in culture; however, GIM-1 acted as an important cytoprotective agent reversing our attenuating MBP effects. (AU)

FAPESP's process: 12/00253-3 - Methylation pattern of the imprinting controlling regions and expression of the H19 and IGF-2 genes and androgenic response in human Sertoli cells after exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TDCC)
Grantee:Wellerson Rodrigo Scarano
Support Opportunities: Regular Research Grants