Pharmacological and transcriptomic characterization of the nitric oxide pathway in aortic rings isolated from the tortoise Chelonoidis carbonaria

Author(s)

Campos, Rafael ^{[1, 2]} ; Oliveira Justo, Alberto Fernando ^[1] ; Jacintho, Felipe Fernandes ^[1] ; Monica, Fabiola Z. ^[1] ; Rojas-Moscoso, Julio Alejandro ^[1] ; Moreno, Ronilson Agnaldo ^[1] ; Napolitano, Mauro ^[3] ; Cogo, Jose Carlos ^[4] ; De Nucci, Gilberto ^{[3, 1]}

Affiliation

^[1] Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, SP - Brazil

^[2] Ceara State Univ UECE, Super Inst Biomed Sci, Fortaleza, Ceara - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[4] Brazil Univ, Fac Biomed Engn, Itaquera - Brazil

• Document type: Journal article
• Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 222, p. 82-89, AUG 2019.
• Web of Science Citations: 0

Abstract

In this study the nitric oxide (NO)-soluble guanylate cyclase (sGC) and phosphodiesterase-5 (PDE-5) pathway was characterized in tortoise Chelonoidis carbonaria aorta. Concentration response curves (CCR) to ATP, ADP, AMP, adenosine and histamine were performed in the presence and absence of L-NAME in aorta pre-contracted with ACh (3 mu M). CCR to SNP, BAY 41-2272 (sGC stimulator), BAY 60-2770 (sGC activator) and tadalafil (PDE-5 inhibitor) were constructed in the presence and absence of ODQ (10 mu M). ATP (pEC(50) 6.1 +/- 0.1), ADP (PECso 6.0 +/- 0.2), AMP (pEC(50) 6.8 +/- 0.1) and histamine (pEC(50) 6.8 +/- 0.12) relaxed Chelonoidis aorta and the addition of L-NAME reduced their efficacy (p < .05). Adenosine effects (pEC(50) 6.6 +/- 0.1) were not changed in the presence of L-NAME. SNP (pEC(50) 7.5 +/- 0.7; Emax 102.2 +/- 2.5%), BAY 41-2272 (pEC(50) 7.3 +/- 0.2; Emax 130.3 +/- 10.2%), BAY 60-2770 (pEC(50) 11.4 +/- 0.1; Emax 130.3 +/- 6.5%) and tadalafil (pEC(50) 6.7 +/- 0.3; Emax 121.3 +/- 15.3%) relaxed Chelonoidis aorta. The addition of ODQ reduced the SNP and tadalafil maximum response (p < .05) and promoted 63 fold right shift on BAY 41-2272 curve. In contrast, no alteration was observed on BAY 60-2770 response. Transcriptomic analysis for eNOS and sGC were found in aorta and brain libraries with high homology when compared with human transcripts. The NO-sGC-PDE-5 is functionally present in Chelonoidis aorta with a functional and genomic similarity to mammalian vessels. Unlike most of mammalian vessels, ACh did not cause endothelium-dependent relaxation in Chelonoidis carbonaria aortic rings. (AU)

• FAPESP's process: 11/11828-4 - Pharmacological, eletrophysiological and morphological characterization of a novel TTX-resistant sodium channel coupled to corpus cavernosum of snakes
• Grantee: Gilberto de Nucci
• Support Opportunities: Research Projects - Thematic Grants