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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laminin-derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells

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Author(s):
Caires-dos-Santos, Livia [1] ; da Silva, Suely V. [1] ; Smuczek, Basilio [1, 2] ; de Siqueira, Adriane S. [1, 3] ; Cruz, Karen S. P. [4, 5, 6] ; Barbuto, Jose Alexandre M. [4] ; Augusto, Taize M. [7, 8] ; Freitas, Vanessa M. [1] ; Carvalho, Hernandes F. [3] ; Jaeger, Ruy G. [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo, SP - Brazil
[2] UNICTR State Univ, Dept Biol, Guarapuava, PR - Brazil
[3] Posit Univ, Sch Dent, Curitiba, Parana - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, SP - Brazil
[5] Univ Fed Alagoas, Fac Nutr, Maceio, AL - Brazil
[6] Univ Fed Alagoas, Inst Hlth & Biol Sci, Cell Biol Lab, Maceio, AL - Brazil
[7] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[8] Sch Med Jundiai, Dept Morphol & Basic Pathol, Jundiai, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Journal of Cellular Physiology; v. 235, n. 1 JUN 2019.
Web of Science Citations: 1
Abstract

Laminin peptides influence cancer biology. We investigated the role of a laminin-derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane-type matrix metalloproteinase 1. Reactive oxygen species generation is also related to invadopodia formation. This prompted us to address whether C16 would induce reactive oxygen species generation in DU145 cells. Quantitative fluorescence and flow cytometry showed that the peptide C16 increased reactive oxygen species in DU145 cells. Furthermore, significant colocalization between Tks5 and reactive oxygen species was observed in C16-treated cells. Results suggested that the peptide C16 increased Tks5 and reactive oxygen species in prostate cancer cells. The role of C16 increasing Tks and reactive oxygen species are novel findings on invadopodia activity. (AU)

FAPESP's process: 09/16150-6 - Androgen regulation, sinalization and cellular interactions in prostate development, physiology and regression
Grantee:Hernandes Faustino de Carvalho
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/03528-0 - Interaction between C16 peptide, derived from laminin-111, and beta1 integrin in breast cells. Related mechanisms and functional consequences
Grantee:Ruy Gastaldoni Jaeger
Support Opportunities: Regular Research Grants
FAPESP's process: 09/17336-6 - Laminin peptides inducing invadopodia in a cell line derived from squamous cell carcinoma: dynamic study by 4D microscopy
Grantee:Adriane Sousa de Siqueira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/07699-1 - Protease ADAMTS1 influencing breast cancer behavior and microenvironment
Grantee:Vanessa Morais Freitas
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/03393-9 - Analysis of laminin cleavage, generating putative bioactive peptides.
Grantee:Ruy Gastaldoni Jaeger
Support Opportunities: Regular Research Grants