Does Prolactin treatment trigger imunoendocrine alterations during experimental T. cruzi infection?

Author(s)

Filipin, Marina Del Vecchio ^[1] ; Brazao, Vania ^[1] ; Santello, Fabricia Helena ^[1] ; Bronzon da Costa, Cassia Mariana ^[1] ; Alonso Toldo, Miriam Paula ^[1] ; de Morais, Fabiana Rossetto ^[1] ; do Prado Junior, Jose Clovis ^[1]

Affiliation

^[1] Univ Sao Paulo, Coll Pharmaceut Sci Ribeirao Preto FCFRP, Ave Cafe S-N, BR-14040903 Sao Paulo - Brazil

• Document type: Journal article
• Source: CYTOKINE; v. 121, SEP 2019.
• Web of Science Citations: 1

Abstract

Prolactin (PRL) is a pleiotropic polypeptide hormone produced by the anterior pituitary gland and negatively controlled by dopamine. Some researchers have associated the immune regulatory functions of PRL with some infectious diseases like Toxoplasma gondii and T. cruzi. This work aimed to analyze the possible immuno-modulatory effects of this hormone through the subcutaneous administration of PRL during the experimental Chagas disease. On the 14th day post-infection (dpi), PRL triggered increased percentages of NK cells in treated infected animals as compared to the infected and untreated ones. For early and late apoptosis, our results showed that in chronically infected groups, PRL counteracted splenocyte apoptosis as revealed by the reduced percentages of both, early and late apoptosis. Reduced percentages of spleen CD4(+) and CD8(+) T cells were detected in infected PRL treated rats (60 days post-infection). Concerning to B cells, a significant increased percentage of these cells was found for all PRL treated infected animals (14th dpi), but no statistically significant alteration was observed on the 60th days post-infection. Furthermore, PRL treatment triggered a significant increase in the percentage of CD4(+) T lymphocytes IFN-gamma producers, while on the 60th dpi, a reduced percentage of IFN-gamma in these cells was observed in prolactin-treated rats compared to infected and untreated ones. Enhanced serum IL-12 levels were detected in infected and PRL treated subjects (60th dpi). Only on 7th day post-infection, the flow cytometric analysis of CFSE-stained CD3(+) T cells showed an enhanced proliferation of polyclonal stimulated T cells in PRL-treated and infected animals. In this study, we demonstrated that PRL can influence many aspects of the immune response during the experimental Chagas' disease, and this substance could be used as a supporting trial along with the conventional drug treatment. (AU)

• FAPESP's process: 10/19895-0 - Immunomodulatory actions of zinc and prolactin during the course of experimental Trypanosoma cruzi infection
• Grantee: José Clóvis do Prado Júnior
• Support Opportunities: Regular Research Grants