Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii

Author(s)

da Silva, Roberta Peres ^[1] ; Longo, V, Larissa G. ; da Cunha, Julia P. C. ^[2] ; Sobreira, Tiago J. P. ^[3] ; Rodrigues, Marcio L. ^{[4, 5]} ; Faoro, Helisson ^[4] ; Goldenberg, Samuel ^[4] ; Alves, Lysangela R. ^[4] ; Puccia, Rosana ^[6]

Affiliation

^[1] Escola Paulista Med Univ Fed Sao Paulo EPM UNIFES, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

^[2] Butantan Inst, Immune Response & Cell Signaling Ctr CeTICS, Lab Especial Ciclo Celular, BR-05503900 Sao Paulo - Brazil

^[3] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 - USA

^[4] Inst Carlos Chagas Fiocruz PR, BR-81350010 Curitiba, Parana - Brazil

^[5] Univ Fed Rio de Janeiro, Inst Microbiol, BR-21941590 Rio De Janeiro - Brazil

^[6] Longo, Larissa G., V, Escola Paulista Med Univ Fed Sao Paulo EPM UNIFES, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

• Document type: Journal article
• Source: CELLS; v. 8, n. 7 JUL 2019.
• Web of Science Citations: 0

Abstract

Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content. (AU)

• FAPESP's process: 13/25950-1 - Expression and regulation of genes related to the cell wall from dimorphic pathogenic fungi under stress conditions
• Grantee: Rosana Puccia
• Support Opportunities: Regular Research Grants