| Full text | |
| Author(s): |
Machado De-Paula, Mirian Michelle
[1]
;
Afewerki, Samson
[2, 3]
;
Viana, Bartolomeu Cruz
[4, 5]
;
Webster, Thomas Jay
[6]
;
Lobo, Anderson Oliveira
[7]
;
Marciano, Fernanda Roberta
[4, 5]
Total Authors: 6
|
| Affiliation: | [1] Univ Vale Paraiba, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 - USA
[3] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 - USA
[4] Univ Fed Piaui, Dept Phys, BR-64049550 Teresina, PI - Brazil
[5] Univ Fed Piaui, Mat Sci & Engn Grad Program, BR-64049550 Teresina, PI - Brazil
[6] Northeastern Univ, Dept Chem Engn, Nanomed Lab, Boston, MA 02115 - USA
[7] Univ Fed Piaui, Dept Mat Engn, Interdisciplinary Lab Adv Mat, BR-64049550 Teresina, PI - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 103, OCT 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Herein, we electrospun ultrathin core-shell fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), gelatin and osteogenic growth peptide (OGP), and evaluated their potential to upregulate human osteoblast cells (hFOB) and to reduce Gram-positive and Gram-negative bacteria. We also evaluated the fiber morphology, chemical structure and peptide delivery efficacy. The employment of core-shell fibers compared to fibers without a core-shell showed improved mechanical strength, comparable to the strength of pure PCL, as well as improved hydrophilicity and wettability. The careful selection of polymer combination and core-shell strategy promoted a controlled and sustained release of OGP. Moreover, increased calcium deposition (CD) (1.3-fold) and alkaline phosphate (ALP) activity was observed when hFOBs were cultivated onto core-shell fibers loaded with OGP after 21 days of culture. Our developed scaffolds were also able to reduce the amount of Pseudomonas aeruginosa (ATCC 25668) bacteria by a factor of two compared to raw PCL without the use of any antibiotics. All of these results demonstrate a promising potential of the developed core-shell electrospun scaffolds based on PCL:PEG:Gelatin:OGP for numerous bone tissue applications. (AU) | |
| FAPESP's process: | 14/16295-2 - Nanofibers produced by electrospinning and rotary jet-spinning with incorporated hydroxyapatite and carbon nanotubes to verify its potential in bone regeneration |
| Grantee: | Mirian Michelle Machado de Paula |
| Support type: | Scholarships in Brazil - Doctorate |