Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity

Author(s)

Machado De-Paula, Mirian Michelle ^[1] ; Afewerki, Samson ^{[2, 3]} ; Viana, Bartolomeu Cruz ^{[4, 5]} ; Webster, Thomas Jay ^[6] ; Lobo, Anderson Oliveira ^[7] ; Marciano, Fernanda Roberta ^{[4, 5]}

Affiliation

^[1] Univ Vale Paraiba, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 - USA

^[3] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 - USA

^[4] Univ Fed Piaui, Dept Phys, BR-64049550 Teresina, PI - Brazil

^[5] Univ Fed Piaui, Mat Sci & Engn Grad Program, BR-64049550 Teresina, PI - Brazil

^[6] Northeastern Univ, Dept Chem Engn, Nanomed Lab, Boston, MA 02115 - USA

^[7] Univ Fed Piaui, Dept Mat Engn, Interdisciplinary Lab Adv Mat, BR-64049550 Teresina, PI - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 103, OCT 2019.
• Web of Science Citations: 0

Abstract

Herein, we electrospun ultrathin core-shell fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), gelatin and osteogenic growth peptide (OGP), and evaluated their potential to upregulate human osteoblast cells (hFOB) and to reduce Gram-positive and Gram-negative bacteria. We also evaluated the fiber morphology, chemical structure and peptide delivery efficacy. The employment of core-shell fibers compared to fibers without a core-shell showed improved mechanical strength, comparable to the strength of pure PCL, as well as improved hydrophilicity and wettability. The careful selection of polymer combination and core-shell strategy promoted a controlled and sustained release of OGP. Moreover, increased calcium deposition (CD) (1.3-fold) and alkaline phosphate (ALP) activity was observed when hFOBs were cultivated onto core-shell fibers loaded with OGP after 21 days of culture. Our developed scaffolds were also able to reduce the amount of Pseudomonas aeruginosa (ATCC 25668) bacteria by a factor of two compared to raw PCL without the use of any antibiotics. All of these results demonstrate a promising potential of the developed core-shell electrospun scaffolds based on PCL:PEG:Gelatin:OGP for numerous bone tissue applications. (AU)

• FAPESP's process: 14/16295-2 - Nanofibers produced by electrospinning and rotary jet-spinning with incorporated hydroxyapatite and carbon nanotubes to verify its potential in bone regeneration
• Grantee: Mirian Michelle Machado de Paula
• Support Opportunities: Scholarships in Brazil - Doctorate