Photodithazine-mediated antimicrobial photodynamic therapy against fluconazole-resistant Candida albicans in vivo

Author(s)

Alves, Fernanda ^[1] ; Carmello, Juliana Cabrini ^[1] ; de Oliveira Mima, Ewerton Garcia ^[1] ; de Souza Costa, Carlos Alberto ^[2] ; Bagnato, Vanderlei Salvador ^[3] ; Pavarina, Ana Claudia ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, Grp Opt, Biophoton Lab, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Medical Mycology; v. 57, n. 5, p. 609-617, JUL 2019.
• Web of Science Citations: 3

Abstract

This study evaluated the efficacy of antimicrobial Photodynamic Therapy (aPDT) against fluconazole-resistant Candida albicans in a murine model of oral candidosis. Mice were inoculated with two clinical isolates (R10, R15) and one reference strain (ATCC) of resistant C. albicans to produce oral candidosis. After inoculation, aPDT mediated by Photodithazine((R)) (PDZ) and LED light was performed. The use of PDZ or light only was also investigated. Additional animals were treated with Nystatin (NYS). Untreated or healthy mice were also evaluated. Microbiological evaluation was performed by recovering C. albicans from the tongue via colony-forming units. Animals were killed 24 hours after treatments, and the tongues were removed for histological analysis. Data were analyzed by one-way ANOVA and Tukey test (P < .05). The results demonstrated that all strains showed the same behavior after aPDT and NYS treatment. A significant reduction in C. albicans viability was achieved after both treatments for R15 and ATCC. No significant reduction was verified for C. albicans R10 submitted to aPDT or NYS. The histological analysis revealed that aPDT did not cause side effects on tissues. aPDT was effective for inactivation of two fluconazole-resistant C. albicans of the three strains evaluated. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/15304-0 - Study in vivo of Photodynamic Therapy mediated by Photodithazione and LED light on Candida albicans fluconazole-resistant
• Grantee: Fernanda Alves Dias de Sousa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/02942-0 - Study in vivo of photodynamic therapy mediated by Photodithazione and led light on Candida albicans fluconazole-resistant
• Grantee: Ana Cláudia Pavarina
• Support Opportunities: Regular Research Grants