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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Learning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection

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Author(s):
Arencibia, Ariel D. [1] ; D'Afonseca, Vivian [1, 2] ; Chakravarthi, Mohan [3, 4] ; Castiglione, Stefano [5]
Total Authors: 4
Affiliation:
[1] Univ Catolica Maule, Ctr Biotecnol Recursos Nat CenBio, Talca - Chile
[2] Univ Catolica Maule, Vicerrectoria Invest & Postgrad VRIP, Talca - Chile
[3] Univ Florida, Dept Agron, Gainesville, FL 32611 - USA
[4] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil
[5] Univ Salerno, Dipartimento Chim & Biol, Salerno - Italy
Total Affiliations: 5
Document type: Review article
Source: ELECTRONIC JOURNAL OF BIOTECHNOLOGY; v. 41, p. 22-29, SEP 2019.
Web of Science Citations: 0
Abstract

We highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, transgenic or genome editing (CRISPR/Cas9). The incidence of undesirable somadonal variations and/or epigenetic changes that might have occurred during in vitro multiplication and regeneration processes, must be carefully analyzed in replicates in field trials. One remarkable challenge is related to the time lapse that selects the modified elite genotypes, because these strategies may spend a variable amount of time before the results are commercialized, where in all the cases it should be take into account the genotype x environment interactions. Furthermore, this combination of techniques can create an encouraging bridge between the public opinion and the community of geneticists who are concerned with plant genetic improvement. In this context, either transgenesis or genomic editing strategies become complementary modern tools to facing the challenges of plant genetic improvement. Their applications will depend on case-by-case analysis, and when possible will necessary associate them to the schemes and bases of classic plant genetic improvement. (C) 2019 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 18/11544-5 - CRISPR/Cas9 based targeted insertion of cystatins in a pre-characterized locus of sugarcane
Grantee:Chakravarthi Mohan
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor