Antimicrobial photodynamic therapy reduces adhesion capacity and biofilm formation of Candida albicans from induced oral candidiasis in mice

Author(s)

Carmello, Juliana Cabrini ^[1] ; Alves, Fernanda ^[1] ; Basso, Fernanda G. ^[2] ; de Souza Costa, Carlos Alberto ^[2] ; Tedesco, Antonio Claudio ^[3] ; Primo, Fernando Lucas ^[4] ; de Oliveira Mima, Ewerton Garcia ^[1] ; Pavarina, Ana Claudia ^[1]

Affiliation

^[1] Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sch Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Dept Physiol & Pathol, Sch Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[3] FECLRP Sao Paulo Univ, Ctr Nanotechnol & Tissue Engineers, Photobiol & Photomed Res Grp, Av Bandeirantes 3 900, BR-14040900 Ribeirao Preto, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Dept Biotechnol & Bioproc, Sch Pharm, Rodovia Araraquara Jau, Km 01 S-N, BR-14800903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 27, p. 402-407, SEP 2019.
• Web of Science Citations: 1

Abstract

Background: : Antimicrobial photodynamic therapy (aPDT) has been considered an alternative therapeutic modality for the treatment of Candida infections. However, most studies are focused mainly on microorganism's inactivation efficiency. Here, we evaluated the efficacy of aPDT mediated by chloro-aluminum phthalocyanine encapsulated in cationic nanoemulsions (ClAlP-NE) to treat oral candidiasis in vivo and its effect on the adhesion and biofilm formation of Candida albicans. Methods: : For this, mice were immunosuppressed and inoculated with C. albicans to produce oral candidiasis. aPDT and Nystatin were applied for 5 successive sessions. Next, the microbiological evaluation was determined (CFU/ml) and the analyses of virulence factors (adhesion capacity and biofilm formation) were performed. Data were analyzed by Two-way ANOVA (alpha = 0.05). Results: : aPDT was as effective as Nystatin reducing 1.4 and 2.0 log(10) of the cell viability (p <= 0.0001), respectively. Both treatments reduced the adhesion capacity and biofilm formation of C. albicans (p <= 0.0001) Conclusion: : ClAlP-NE-mediated aPDT was effective in reducing the virulence factors of C. albicans and also to treat induced oral candidiasis in mice. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 12/17468-2 - Effect of Antimicrobial Photodynamic Therapy in a murine model of oral candidosis. In vivo evaluation of a Photodithazine hydrogel and Aluminum-Chlorine-Phthalocyanine cationic nanoemulsion.
• Grantee: Juliana Cabrini Carmello
• Support Opportunities: Scholarships in Brazil - Doctorate