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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expressed proteins and activated pathways in conditioned embryo culture media from IVF patients are diverse according to infertility factors

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Author(s):
Bonetti, Tatiana C. S. [1] ; Haddad, Debora C. M. [2] ; Donningues, Thais S. [1, 3] ; Alegretti, Jose Roberto [3] ; Motta, Eduardo L. A. [1, 3] ; Seeley, Kent [4] ; Silva, Ismael D. C. G. [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP EPM, Escola Paulista Med, Dept Ginecol, Discipline Ginecol Endocrinol, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo UNIFESP EPM, Escola Paulista Med, Dept Urol, Setor Integrad Reprod Humana, Sao Paulo, SP - Brazil
[3] Huntington Med Reprod, Sao Paulo, SP - Brazil
[4] Univ S Florida, CDDI, Prote & Mass Spectrometry Facil, Tampa, FL - USA
Total Affiliations: 4
Document type: Journal article
Source: JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA; v. 23, n. 4, p. 352-360, OCT-DEC 2019.
Web of Science Citations: 0
Abstract

Objective: Objective: Given that the embryo culture medium secretome reflects the embryo development, we hypothesize that protein profiles are affected according to infertility factors, which can be responsible for detrimental embryonic developmental competence. The aim of this study was to screen the protein profile of conditioned embryo culture media in patients presenting deep infiltrating endometriosis (ENDO) and polycystic ovarian syndrome (PCOS) undergoing IVF, by proteomics approaches. The control group was constituted by tubal factor patients. Methods: Patients underwent in vitro fertilization (IVF) treatment as routine and oocytes were fertilized by ICSI. The embryos were group cultured until day 3 of development, and after transfer the culture media were collected. For the proteomics analysis, two pools of samples were prepared for groups CONTROL and PCOS, and 4 pools of samples for group DIE. Samples were prepared to deplete high abundant proteins and followed evaluated by high throughput proteomics approach. Results: The embryonic organ and tissue development were physiological functions activated, based on proteins identified in the 3 study groups of samples. The samples coming from DIE patients presented a high calcium activity and on the other hand, embryos coming from PCOS patients showed a decreased calcium action. Other pathways as grow factors through the EGF signaling pathway overexpressed in ENDO culture medium and protein kinase A in PCOS were also observed. Conclusions: Proteomic embryonic secretome will advance our knowledge of early embryogenesis and additionally could lead to improved selection of embryos for transfer warrants further investigation. (AU)

FAPESP's process: 10/51873-6 - Proteomic profile of follicular fluid, granulosa cells and embryo culture medium of patients undergoing in vitro fertilization cycles: identification of factors evolved at oocyte quality and embryo development in patients with different infertility etiologies
Grantee:Ismael Dale Cotrim Guerreiro da Silva
Support Opportunities: Regular Research Grants