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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and Validation of Shiga Toxin-Producing Escherichia coli Immunodiagnostic Assay

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Author(s):
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Silva, Miriam A. [1] ; Santos, Anna Raquel R. [1, 2] ; Rocha, Leticia B. [1] ; Caetano, Bruna A. [1] ; Mitsunari, Thais [1] ; Santos, Luanda I. [1] ; Polatto, Juliana M. [1] ; Horton, Denise S. P. Q. [1] ; Guth, Beatriz E. C. [3] ; dos Santos, Luis Fernando [4] ; Piazza, Roxane M. F. [1]
Total Authors: 11
Affiliation:
[1] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo - Brazil
[2] Univ Fed Minas Gerais, Ctr Tecnol Vacinas, BR-31270901 Belo Horizonte, MG - Brazil
[3] Univ Fed Sao Paulo, Dept Microbiol, Escola Paulista Med, Imunol, Parasitol, BR-04023062 Sao Paulo - Brazil
[4] Adolfo Lutz Inst, Ctr Bacteriol, BR-01246000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MICROORGANISMS; v. 7, n. 9 SEP 2019.
Web of Science Citations: 0
Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC) and its subgroup enterohemorrhagic E. coli are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal failure in children. Early diagnosis is essential for clinical management, as an antibiotic treatment in STEC infections is not recommended. Previously obtained antibodies against Stx(1) and Stx(2) toxins were employed to evaluate the sensitivity and specificity of the latex Agglutination test (LAT), lateral flow assay (LFA), and capture ELISA (cEIA) for STEC detection. The LAT (mAb Stx(1) plus mAb stx(2)) showed 99% sensitivity and 97% specificity. Individually, Stx(1) antibodies showed 95.5% and 94% sensitivity and a specificity of 97% and 99% in the cEIA and LFA assay, respectively. Stx(2) antibodies showed a sensitivity of 92% in both assays and a specificity of 100% and 98% in the cEIA and LFA assay, respectively. These results allow us to conclude that we have robust tools for the diagnosis of STEC infections. (AU)

FAPESP's process: 12/02618-9 - Validation of an immunochromatographic assay for the diagnosis of diarrheagenic Escherichia coli
Grantee:Anna Raquel Ribeiro dos Santos
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/06589-6 - Immunochromatographic assay as diagnostic tool for neglected diseases: studies of improvement, scheduling and feasibility
Grantee:Roxane Maria Fontes Piazza
Support type: Regular Research Grants