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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intestinal microbiota of broilers submitted to feeding restriction and its relationship to hepatic metabolism and fat mass: Fast-growing strain

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Author(s):
Lunedo, Raquel [1] ; Furlan, Luiz R. [2] ; Fernandez-Alarcon, Miguel F. [1] ; Squassoni, Gustavo H. [2] ; Campos, Daniel M. B. [3] ; Perondi, Dani [1] ; Macari, Marcos [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci, Jaboticabal, SP - Brazil
[2] Sao Paulo State Univ Unesp, Aquaculture Ctr Unesp, Jaboticabal, SP - Brazil
[3] Fed Univ Sao Carlos UFSCar, Campus Lagoa Sino, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION; v. 103, n. 4, p. 1070-1080, JUL 2019.
Web of Science Citations: 0
Abstract

The present study was conducted to verify how feed restriction affects gut microbiota and gene hepatic expression in broiler chickens and how these variables are related to body weight gain. For the experiment, 21-d-old Cobb500 (TM) birds were distributed in a completely randomized experimental design with three treatments: T1. Control (ad libitum-3.176 Mcal/kg ME-metabolizable energy-and 19% CP-crude protein); T2. Energetic restriction (2.224 Mcal/kg ME and 19% CP) from 22 to 42 days with consumption equivalent to control; T3. Quantitative restriction (70% restriction, i.e., restricted broilers ingested only 30% of the quantity consumed by the control group-3.176 Mcal/kg ME and 19% CP) for 7 days, followed by refeeding ad libitum from 28 to 42 days. Ileum and caecum microbiota collections were made at 21, 28 and 42 days of age. Hepatic tissue was collected at 28 and 42 days old for relative gene expression analyses. At 43-d-old, body composition was quantified by DXA (Dual-energy X-ray Absorptiometry). Both feed restriction programmes decreased Lactobacillus and increased Enterococcus and Enterobacteriaceae counts. No differences were found in the refeeding period. Energetic restriction induced the expression of CPT1-A (Carnitine palmitoyltransferase 1A) gene, and decreased body fat mass. Quantitative feed restriction increased lipogenic and decreased lipolytic gene expression. In the refeeding period, CPT1-A gene expression was induced, without changing the broilers body composition. Positive associations were found between BWG (Body Weight Gain) and Lactobacillus and Clostridium cluster IV groups, and negatively associations with Enterobacteriaceae and Enterococcus bacterial groups. In conclusion, differences found in microbiota were similar between the two feed restriction programmes, however, hepatic gene expression differences were only found in quantitative restriction. Higher counts of Lactobacillus and Clostridium cluster IV groups in ileum are likely to be related to better broiler performance and low expression of lipogenic genes. (AU)

FAPESP's process: 13/05924-6 - Effect of energy restriction and genetic group on the microbiota of the gastrointestinal tract of broilers.
Grantee:Raquel Lunedo
Support Opportunities: Scholarships in Brazil - Doctorate