Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus

Author(s)

de Souza de Azevedo, Pamela Oliveira ^[1] ; de Azevedo, Hernando Fernandes ^[1] ; Figueroa, Elias ^[2] ; Converti, Attilio ^[3] ; Manuel Dominguez, Jose ^[4] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Ave Lineu Prestes 580, BR-05508900 Sao Paulo - Brazil

^[2] Univ Catolica Temuco, Fac Recursos Nat, Dept Ciencias Biol & Quim, Nucleo Invest Prod Alimentaria, Temuco - Chile

^[3] Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, I-16145 Genoa - Italy

^[4] Univ Vigo, Fac Sci, Dept Chem Engn, Campus Ourense, As Lagoas S-N, Orense 32004 - Spain

• Document type: Journal article
• Source: MOLECULAR BIOLOGY REPORTS; v. 46, n. 5, p. 4883-4891, OCT 2019.
• Web of Science Citations: 0

Abstract

To improve bacteriocin-like inhibitory substance (BLIS) production by Pediococcus pentosaceus ATCC 43200, the influence of pH as well as the addition of sugars-either prebiotic (inulin) or not (sucrose)-on its metabolism were investigated. This strain was grown at pH 5.0 or 6.0 either in glucose-based MRS medium (control) or after addition of 0.5, 1.0 or 1.5% (w/w) sucrose and inulin (GSI-MRS) in the same percentages. In the control medium at pH 5.0, cell mass concentration after 48 h of fermentation (X-max = 2.26 g/L), maximum specific growth rate (mu(max) = 0.180 h(-1)) and generation time (T-g = 3.84 h) were statistically coincident with those obtained in supplemented media. At pH 6.0 some variations occurred in these parameters between the control medium (X-max = 2.68 g/L; mu(max) = 0.32 h(-1); T-g = 2.17 h) and the above supplemented media (X-max = 1.90, 2.52 and 1.86 g/L; mu(max) = 0.26, 0.33 and 0.32 h(-1); T-g = 2.62, 2.06 and 2.11 h, respectively). Lactate production was remarkable at both pH values (13 and 16 g/L) and improved in all supplemented media, being 34 and 54% higher than in their respective control media, regardless of the concentration of these ingredients. Cell-free supernatant of the fermented control medium at pH 5.0 displayed an antimicrobial activity against Enterococcus 101 5.3% higher than that at pH 6.0 and even 20% higher than those of all supplemented media, regardless of the concentration of supplements. BLIS production was favored either at pH 5.0 or in the absence of any additional supplements, which were able, instead, to stimulate growth and lactate production by P. pentosaceus. (AU)

• FAPESP's process: 18/04563-3 - Biotechnological production of antimicrobial compounds of interest to the pharmaceutical, veterinary and food industries
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• FAPESP's process: 18/04385-8 - Construction of a pediocin-producing recombinant lineage from agroindustrial waste
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Regular Research Grants