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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Short-term high-fat diet affects macrophages inflammatory response, early signs of a long-term problem

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Author(s):
Santos, Ed Wilson [1] ; Oliveira, Dalila Cunha [1] ; Hastreiter, Araceli [1] ; Silva, Graziela Batista [1] ; de Oliveira Beltran, Jackeline Soares [1] ; Rogero, Marcelo Macedo [2] ; Fock, Ricardo Ambrosio [1] ; Borelli, Primavera [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 55, 2019.
Web of Science Citations: 0
Abstract

Obesity is a chronic inflammatory disease that affects millions of people worldwide. Most studies observe the effects of a high-fat diet (HFD) in 10-12 weeks. This work investigated the effects induced by a HFD administered for 6 weeks on the nutritional status of mice and some aspects of the inflammatory response in mouse peritoneal macrophages. Male Swiss Webster mice, 2-3 months of age, were fed a control diet or HFD for 6 weeks. After this period, the mice were euthanized, and peritoneal macrophages were collected for immunoassays and assessment of biochemical parameters. A HFD was associated with increased cholesterol, insulin resistance, C-reactive protein (CRP), leptin, and serum resistin levels. Lipopolysaccharide (LPS)- stimulated adipocyte cultures of animals subjected to a HFD showed increased production of proinflammatory cytokines such as tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1), and interleukin-6 (IL-6). However, peritoneal macrophages of the HFD group showed no changes in the levels of these cytokines. LPS-stimulated peritoneal macrophages from HFD-treated animals showed a reduction in mRNA expression of TNF-alpha and IL-6, as well as a decrease in expression of the transcription factor nuclear factor-kappa B (NF-kB). In conclusion, HFD treatment for 6 weeks induces similar signs to metabolic syndrome and decreases the capacity of peritoneal macrophages to develop an appropriate inflammatory response to a bacterial component. (AU)

FAPESP's process: 12/24505-1 - Study of the function of peritoneal macrophages of Swiss mice submitted to a low protein diet or high fat diet
Grantee:Primavera Borelli Garcia
Support Opportunities: Regular Research Grants