| Full text | |
| Author(s): Show less - |
Yamil Chain, Cecilia
[1]
;
Pires Souto, Denio Emanuel
[2]
;
Laura Sbaraglini, Maria
[3]
;
Labriola, Carlos A.
[4]
;
Daza Millone, Maria Antonieta
[1]
;
Alejandro Ramirez, Eduardo
[1]
;
Sebastian Cisneros, Jose
[1]
;
Lopez-Albizu, Constanza
[5]
;
Scollo, Karenina
[5]
;
Kubota, Lauro T.
[6]
;
Mariano Ruiz, Andres
[5]
;
Elena Vela, Maria
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Nacl La Plata, CONICET, Inst Invest Fisicoquim Teor & Aplicadas INIFTA, Diagonal 113 & 64 S-N, RA-1900 La Plata, Buenos Aires - Argentina
[2] Fed Univ Parana UFPR, Dept Chem, Ave Coronel Francisco Heraclito Santos, BR-81530000 Curitiba, Parana - Brazil
[3] Univ Nacl La Plata, Fac Ciencias Exactas, Dept Ciencias Biol, Lab Invest & Desarrollo Bioactivos LIDeB, Calle 47 200-236, ARB1900AJJ, La Plata, Buenos Aires - Argentina
[4] Univ Nacl Buenos Aires, CONICET, IIBBA, Fdn Inst Leloir, Av Patricias Argentinas 435, AR C1405BWE, Buenos Aires, DF - Argentina
[5] Adm Nacl Inst & Lab Salud ANLIS, Inst Nacl Parasitol Dr Mario Fatala Chaben, Ave Paseo Colon 568, C1063ACR, Buenos Aires, DF - Argentina
[6] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, Rua Josue de Castro S-N, Cidade Univ Barao Geraldo, BR-13083861 Campinas, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | ACS INFECTIOUS DISEASES; v. 5, n. 11, p. 1813-1819, NOV 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
trans-Sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. The aim of the present study is to assess the possibility of using cruzipain and the catalytic domain of trans-sialidase in a Surface Plasmon Resonance-based immunosensor for the diagnosis of chronic Chagas disease. Immunoassays carried out with canine sera verified that cruzipain allows the detection of anti-Trypanosoma cruzi antibodies whereas recombinant trans-sialidase did not yield specific detections, due to the high dilutions of serum used in the immunoassays that hinder the possibility to sense the specific low titer antibodies. The developed cruzipain-based biosensor, whose price per assay is comparable to a commercial enzyme-linked immunosorbent assay (ELISA), was successfully applied for the rapid quantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISA. (AU) | |
| FAPESP's process: | 17/26058-6 - Characterization of interactions in the protein quality system for the understanding of its role in the proteostasis maintenance |
| Grantee: | Dênio Emanuel Pires Souto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |