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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Performance of CDC Trioplex qPCR during a dengue outbreak in Brazil

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Author(s):
Colombo, Tatiana Elias [1, 2] ; Versiani, Alice Freitas [1] ; Dutra, Karina Rocha [1] ; Dias Rubiato, Julia Guimaraes [2] ; Galvao, Tayna Manfrin [2] ; Negri Reis, Andreia Francesli [3] ; Nogueira, Mauricio Lacerda [1]
Total Authors: 7
Affiliation:
[1] Fac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Paulista UNIP, Sao Jose Do Rio Preto, SP - Brazil
[3] Prefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Clinical Virology; v. 121, DEC 2019.
Web of Science Citations: 0
Abstract

Background: In recent years real-time reverse transcription polymerase chain reaction (real-time RT-PCR) has become a leading technique for nucleic acid detection and quantification of flaviviruses, including Dengue virus (DENV). Trioplex real-time RT-PCR has the advantages of providing the concurrent detection of Zika virus (ZIKV), DENV, and Chikungunya virus (CHIKV) RNA in human serum. Objective: This study sought to compare the sensitivity and specificity of the Trioplex real-time RT-PCR assay to those provided by CDC DENV TaqMan (R) RT-qPCR assay and conventional PCR when used for DENV detection in the context of a dengue epidemic. Study design: We analyzed 1656 serum samples from symptomatic patients with acute febrile disease for 5 days less between December 2018 and May 2019. The samples were tested using the various PCR-based assays. Results: Of the 1656 serum samples analyzed, 713 (43%) were laboratory-confirmed as arboviruses: 99.86% (712/713) were confirmed as DENV and 0.14% (1/713) were confirmed as ZIKV. Next, 590 samples were selected, and of these, 331 samples (56.1%) were determined to be positive (Ct < 38) and 259 samples (43.9%) were determined to be negative (Ct > 38) using the Trioplex real-time RT-PCR assay. The multiplex method found that the test exhibits 95% sensitivity and 100% specificity. Conclusion: This evaluation demonstrates the capacity of the Trioplex real-time RT-PCR assay to detect DENV at a high sensitivity and specificity in a geographic area with a current dengue outbreak and a lower co-circulation of other arboviruses - such as ZIKV and CHIKV, and the results prove it's applicability as clinical screening test that can serve as a confirmatory test. (AU)

FAPESP's process: 18/17647-0 - Development of a peptide-based serologic multiplex diagnostic nanodevices to differentially identify Dengue and Zika infections
Grantee:Alice Freitas Versiani
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018
Grantee:Maurício Lacerda Nogueira
Support Opportunities: Research Projects - Thematic Grants