| Full text | |
| Author(s): |
Colombo, Tatiana Elias
[1, 2]
;
Versiani, Alice Freitas
[1]
;
Dutra, Karina Rocha
[1]
;
Dias Rubiato, Julia Guimaraes
[2]
;
Galvao, Tayna Manfrin
[2]
;
Negri Reis, Andreia Francesli
[3]
;
Nogueira, Mauricio Lacerda
[1]
Total Authors: 7
|
| Affiliation: | [1] Fac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Paulista UNIP, Sao Jose Do Rio Preto, SP - Brazil
[3] Prefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Journal of Clinical Virology; v. 121, DEC 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: In recent years real-time reverse transcription polymerase chain reaction (real-time RT-PCR) has become a leading technique for nucleic acid detection and quantification of flaviviruses, including Dengue virus (DENV). Trioplex real-time RT-PCR has the advantages of providing the concurrent detection of Zika virus (ZIKV), DENV, and Chikungunya virus (CHIKV) RNA in human serum. Objective: This study sought to compare the sensitivity and specificity of the Trioplex real-time RT-PCR assay to those provided by CDC DENV TaqMan (R) RT-qPCR assay and conventional PCR when used for DENV detection in the context of a dengue epidemic. Study design: We analyzed 1656 serum samples from symptomatic patients with acute febrile disease for 5 days less between December 2018 and May 2019. The samples were tested using the various PCR-based assays. Results: Of the 1656 serum samples analyzed, 713 (43%) were laboratory-confirmed as arboviruses: 99.86% (712/713) were confirmed as DENV and 0.14% (1/713) were confirmed as ZIKV. Next, 590 samples were selected, and of these, 331 samples (56.1%) were determined to be positive (Ct < 38) and 259 samples (43.9%) were determined to be negative (Ct > 38) using the Trioplex real-time RT-PCR assay. The multiplex method found that the test exhibits 95% sensitivity and 100% specificity. Conclusion: This evaluation demonstrates the capacity of the Trioplex real-time RT-PCR assay to detect DENV at a high sensitivity and specificity in a geographic area with a current dengue outbreak and a lower co-circulation of other arboviruses - such as ZIKV and CHIKV, and the results prove it's applicability as clinical screening test that can serve as a confirmatory test. (AU) | |
| FAPESP's process: | 18/17647-0 - Development of a peptide-based serologic multiplex diagnostic nanodevices to differentially identify Dengue and Zika infections |
| Grantee: | Alice Freitas Versiani |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018 |
| Grantee: | Maurício Lacerda Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |