| Full text | |
| Author(s): |
Jimenez-Merino, Juan
[1, 2]
;
de Abreu, Isadora Santos
[3, 4]
;
Hiebert, Laurel S.
[1, 2]
;
Allodi, Silvana
[3, 4]
;
Tiozzo, Stefano
[5]
;
De Barros, Cintia M.
[6]
;
Brown, Federico D.
[7, 1, 2]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biociencias, Dept Zool, Rua Matao, Trav 14, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Ctr Biol Marinha CEBIMar, Sao Paulo, SP - Brazil
[3] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Neurobiol Comparat & Desenvolvimento, Posgrad Ciencias Biol Fisiol, Rio De Janeiro, RJ - Brazil
[4] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Posgrad Ciencias Morfol, Rio De Janeiro, RJ - Brazil
[5] Sorbonne Univ, Lab Biol Dev Villefranche Sur Mer LBDV, CNRS, F-06230 Paris - France
[6] Univ Fed Rio de Janeiro, Lab Integrad Morfol Nucl Ecol & Desenvolvimento S, NUPEM, Macae, RJ - Brazil
[7] Inst Nacl Ciencia & Tecnol Estudos Interdisciplin, Salvador, BA - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | EVODEVO; v. 10, n. 1 NOV 25 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Background In various ascidian species, circulating stem cells have been documented to be involved in asexual reproduction and whole-body regeneration. Studies of these cell population(s) are mainly restricted to colonial species. Here, we investigate the occurrence of circulating stem cells in the solitary Styela plicata, a member of the Styelidae, a family with at least two independent origins of coloniality. Results Using flow cytometry, we characterized a population of circulating putative stem cells (CPSCs) in S. plicata and determined two gates likely enriched with CPSCs based on morphology and aldehyde dehydrogenase (ALDH) activity. We found an ALDH + cell population with low granularity, suggesting a stem-like state. In an attempt to uncover putative CPSCs niches in S. plicata, we performed a histological survey for hemoblast-like cells, followed by immunohistochemistry with stem cell and proliferation markers. The intestinal submucosa (IS) showed high cellular proliferation levels and high frequency of undifferentiated cells and histological and ultrastructural analyses revealed the presence of hemoblast aggregations in the IS suggesting a possible niche. Finally, we document the first ontogenetic appearance of distinct metamorphic circulatory mesenchyme cells, which precedes the emergence of juvenile hemocytes. Conclusions We find CPSCs in the hemolymph of the solitary ascidian Styela plicata, presumably involved in the regenerative capacity of this species. The presence of proliferating and undifferentiated mesenchymal cells suggests IS as a possible niche. (AU) | |
| FAPESP's process: | 19/06927-5 - Forging technical breakthroughs for colonial ascidian developmental biology research: transgenesis and mutagenesis in Botryllus schlosseri |
| Grantee: | Federico David Brown Almeida |
| Support Opportunities: | Scholarships abroad - Research |
| FAPESP's process: | 16/07607-6 - Circulatory stem cells of styelid ascidians |
| Grantee: | Juan Jiménez Merino |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 15/50164-5 - Stem cells, regeneration, and the evolution of coloniality in ascidians |
| Grantee: | Federico David Brown Almeida |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/14052-8 - Origins of coloniality: Developmental mechanisms and directionality of colonial coalescence in ascidians |
| Grantee: | Laurel Sky Hiebert |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/05923-3 - Cells and tissues of dormancy in ascidians: underlying developmental mechanisms and evolutionary origins |
| Grantee: | Laurel Sky Hiebert |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |