Eglerisine, a Novel Sesquiterpenoid Tropolone from Dulacia egleri with Antiproliferative Effect against an Acute Myeloid Leukemia Lineage

Author(s)

de Novais, Leice M. R. ^[1] ; Ferreira, Luiz F. ^[1] ; de Sousa, Jr., PauloT. ^[1] ; Ribeiro, Tereza A. N. ^[1] ; Jacinto, Marcos J. ^[1] ; dos Santos, Carlos H. C. ^[2] ; de Carvalho, Mario G. ^[2] ; Torquato, Heron F. V. ^{[3, 4]} ; Paredes-Gamero, Edgar J. ^{[3, 5]} ; Silva, Virginia C. P. ^[1]

Affiliation

^[1] Univ Fed Mato Grosso, Dept Quim, Cuiaba, MT - Brazil

^[2] Univ Fed Rural Rio de Janeiro, Dept Quim, Seropedica, RJ - Brazil

^[3] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP - Brazil

^[4] Braz Cubas Educ, Fac Farm, Mogi Das Cruzes, SP - Brazil

^[5] Univ Fed Mato Grosso do Sul, Fac Ciencias Farmaceut Alimentos & Nutr FACFAN, Campo Grande, MS - Brazil

• Document type: Journal article
• Source: Planta Medica; v. 86, n. 1, p. 55-60, JAN 2020.
• Web of Science Citations: 0

Abstract

Chemical investigation of the stems of Dulacia egleri resulted in the isolation of eglerisine ( 1 ), a compound with a rare sesquiterpenoid tropolone skeleton. Its structure was determined by analysis of spectrometric and spectroscopic data, including HRESIMS, 1D, and 2D NMR. The antiproliferative effects of eglerisine were tested in human leukemia lineages. In the Kasumi-1 lineage, an acute myeloid leukemia cell line, eglerisine reduced cell metabolism, as determined by the resazurin assay. Eglerisine did not induce cell death by either apoptotic or necrotic mechanisms. However, a reduction of the absolute number of cells was observed. Eglerisine induced cell cycle arrest after 72 h of treatment by phosphorylation of H2AX histone, reducing the S phase and increasing the G2 phase of the cell cycle. (AU)

• FAPESP's process: 16/18990-5 - Studies of cellular and molecular mechanisms of tumor cell death induced by antimicrobial peptides
• Grantee: Edgar Julian Paredes-Gamero
• Support Opportunities: Regular Research Grants