| Full text | |
| Author(s): |
Fernandez-Silva, Frank S.
[1]
;
Schulz, Mariane L.
[2]
;
Alves, Ingrid Reale
[1]
;
Freitas, Rubia R.
[1]
;
da Rocha, Raquel Paes
[1]
;
Lopes-Kulishev, Carina O.
[1]
;
Medeiros, Marisa H. G.
[2]
;
Galhardo, Rodrigo S.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil
[2] Univ S ao Paulo, Inst Chem, Dept Biochem, S ao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Environmental and Molecular Mutagenesis; v. 61, n. 2 OCT 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
8-oxo-7,8-dihydroguanine, commonly referred to as 8-oxoG, is considered one of the most predominant oxidative lesions formed in DNA. Due to its ability to pair with adenines in its syn configuration, this lesion has a strong mutagenic potential in both eukaryotes and prokaryotes. Escherichia coli cells are endowed with the GO system, which protects them from the mutagenic properties of this lesion when formed both in cellular DNA and the nucleotide pool. MutY and MutM (Fpg) DNA glycosylases are crucial components of the GO system. A strong mutator phenotype of the Escherichia coli mutM mutY double mutant underscores the importance of 8-oxoG repair for genomic stability. Here, we report that in Caulobacter crescentus, a widely studied alpha-proteobacterium with a GC-rich genome, the combined lack of MutM and MutY glycosylases produces a more modest mutator phenotype when compared to E. coli. Genetic analysis indicates that other glycosylases and other repair pathways do not act synergistically with the GO system for spontaneous mutation prevention. We also show that there is not a statistically significant difference in the spontaneous levels 8-oxodGuo in E. coli and C. crescentus, suggesting that other yet to be identified differences in repair or replication probably account for the differential importance of the GO system between these two species. Environ. Mol. Mutagen. 2019. (c) 2019 Wiley Periodicals, Inc. (AU) | |
| FAPESP's process: | 14/15982-6 - Consequences of repair deficiencies in damaged genome |
| Grantee: | Carlos Frederico Martins Menck |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |