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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alendronate inhibits osteocyte apoptosis and inflammation via IL-6, inhibiting bone resorption in periapical lesions of ovariectomized rats

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Author(s):
Silva, R. A. B. [1] ; Sousa-Pereira, A. P. [2] ; Lucisano, M. P. [1] ; Romualdo, P. C. [1] ; Paula-Silva, F. W. G. [1] ; Consolaro, A. [3] ; Silva, L. A. B. [1] ; Nelson-Filho, P. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Dent, Ribeirao Preto - Brazil
[2] State Univ Amazonas, Manaus, Amazonas - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Bauru - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Endodontic Journal; v. 53, n. 1 SEP 2019.
Web of Science Citations: 3
Abstract

Aim To evaluate the effect of alendronate (ALN) on the development of periapical lesions induced in ovariectomized rats. Methodology Twenty-five rats were divided into three groups: sham (control), ovariectomy (OVX) and OVX + ALN. One day after OVX, animals from the OVX + ALN group received the medication via gavage. After 9 weeks, the first molars of all animals were submitted to periapical lesion induction. After 21 days, the animals were euthanized. Femurs were analysed for bone mineral density. The blocks of bone tissue containing the mandibular first molars were submitted to histotechnical processing and staining with haematoxylin and eosin (HE) for periapical lesion analysis under conventional microscopy. At the same time, the morphometric analysis of the periapical lesion area was performed in the fluorescence mode, as well as the histoenzimology for the quantification of osteoclasts and 4 `-6-diamidino-2-phenylindole staining for the quantification of apoptotic osteocytes. In addition, the first maxillary molars were used for analysis of the gene expression of proinflammatory cytokines (IL-1 beta, IL-6 and TNF-alpha) and osteoclastogenesis markers (RANKL/OPG). The results were submitted to ANOVA and Kruskal-Wallis tests and Tukey and Dunn post-tests (significance level of 5%). Results Ovariectomy reduced bone mineral density of the femur, and treatment with ALN was able to prevent bone loss (P < 0.001). Regarding the microscopic analysis of the periapical region, the sham and OVX + ALN groups had moderately increased periodontal ligament and inflammatory infiltrate, while the OVX group had these parameters increased intensely. The periapical lesions of the OVX group were significantly larger in area in comparison to the other groups (P < 0.001). The OVX group had the largest amount of apoptotic osteocytes, and ALN was able to prevent the apoptosis of these cells, in addition to significantly reducing IL-6 expression (P < 0.05). OVX and ALN had no effect on RANKL/OPG expression and did not influence the number of osteoclasts around the periapical lesion (P > 0.05). Conclusion The hypoestrogenic condition induced by OVX aggravated bone resorption, inducing the death of osteocytes and provoking larger periapical lesions. ALN treatment inhibited osteocyte apoptosis and inflammation via IL-6, inhibiting bone resorption in periapical lesions of ovariectomized rats. (AU)

FAPESP's process: 14/13238-8 - Evaluation of periapical lesions induced in female osteoporotic rats subjected or not to treatment with bisphosphonate or cathepsin K inhibitor
Grantee:Paulo Nelson Filho
Support Opportunities: Regular Research Grants
FAPESP's process: 13/18231-9 - Evaluation of induced apical periodontitis in osteoporotic mice submitted or not to treatment with alendronate
Grantee:Priscilla Coutinho Romualdo
Support Opportunities: Scholarships in Brazil - Doctorate