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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor

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Author(s):
Lamarca, Rafaela Silva [1, 2] ; Dorledo de Faria, Ricardo Adriano [1, 3] ; Boldrin Zanoni, Maria Valnice [2] ; Nalin, Marcelo [4] ; Feitosa de Lima Gomes, Paulo Clairmont [2] ; Messaddeq, Younes [4, 1]
Total Authors: 6
Affiliation:
[1] Univ Laval, COPL, Quebec City, PQ G1V0A6 - Canada
[2] Sao Paulo State Univ, Natl Inst Alternat Technol Detect Toxicol Evaluat, UNESP, Inst Chem, POB 355, BR-14800900 Araraquara, SP - Brazil
[3] Univ Fed Minas Gerais, Dept Chem Engn, BR-31270901 Belo Horizonte, MG - Brazil
[4] Sao Paulo State Univ UNESP, Inst Chem, POB 355, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: RSC ADVANCES; v. 10, n. 4, p. 1838-1847, JAN 12 2020.
Web of Science Citations: 0
Abstract

In this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Universite Laval residences (Quebec, Canada) through the load transfer resistance (R-ct) using {[}Fe(CN)(6)](3-/4-) as a redox probe, and the average CIP concentration was found to be 2.90 x 10(-4) mu g mL(-1). The observed R-ct changes presented a linear relationship from CIP concentrations of 10(-5) to 1.0 mu g mL(-1), with detection and quantification limits of 2.50 x 10(-6) and 7.90 x 10(-6) mu g mL(-1), respectively. The immunosensor presented high selectivity and repeatability, as well as a good recovery rate in wastewater samples (97%). Significant interference with other compounds was not observed. The proposed method requires only 30 mu L of sample without the use of organic solvents or preceding sample preparation and/or extraction techniques. Moreover, the method is fast: only 20 min of incubation followed by 2 min of analysis time was sufficient to obtain the CIP concentration. The method's estimated cost is U\$ 2.00 per sample. (AU)

FAPESP's process: 18/22393-8 - Biochar applied in the removal of emerging contaminants present in wastewater
Grantee:Paulo Clairmont Feitosa de Lima Gomes
Support type: Regular Research Grants
FAPESP's process: 15/22828-6 - Pushing the boundaries of optical fibers: from photonics to optogenetics and environmental monitoring
Grantee:Younes Messaddeq
Support type: Research Projects - SPEC Program
FAPESP's process: 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass
Grantee:Edgar Dutra Zanotto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC