| Full text | |
| Author(s): |
Lamarca, Rafaela Silva
[1, 2]
;
Dorledo de Faria, Ricardo Adriano
[1, 3]
;
Boldrin Zanoni, Maria Valnice
[2]
;
Nalin, Marcelo
[4]
;
Feitosa de Lima Gomes, Paulo Clairmont
[2]
;
Messaddeq, Younes
[4, 1]
Total Authors: 6
|
| Affiliation: | [1] Univ Laval, COPL, Quebec City, PQ G1V0A6 - Canada
[2] Sao Paulo State Univ, Natl Inst Alternat Technol Detect Toxicol Evaluat, UNESP, Inst Chem, POB 355, BR-14800900 Araraquara, SP - Brazil
[3] Univ Fed Minas Gerais, Dept Chem Engn, BR-31270901 Belo Horizonte, MG - Brazil
[4] Sao Paulo State Univ UNESP, Inst Chem, POB 355, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | RSC ADVANCES; v. 10, n. 4, p. 1838-1847, JAN 12 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
In this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Universite Laval residences (Quebec, Canada) through the load transfer resistance (R-ct) using {[}Fe(CN)(6)](3-/4-) as a redox probe, and the average CIP concentration was found to be 2.90 x 10(-4) mu g mL(-1). The observed R-ct changes presented a linear relationship from CIP concentrations of 10(-5) to 1.0 mu g mL(-1), with detection and quantification limits of 2.50 x 10(-6) and 7.90 x 10(-6) mu g mL(-1), respectively. The immunosensor presented high selectivity and repeatability, as well as a good recovery rate in wastewater samples (97%). Significant interference with other compounds was not observed. The proposed method requires only 30 mu L of sample without the use of organic solvents or preceding sample preparation and/or extraction techniques. Moreover, the method is fast: only 20 min of incubation followed by 2 min of analysis time was sufficient to obtain the CIP concentration. The method's estimated cost is U\$ 2.00 per sample. (AU) | |
| FAPESP's process: | 18/22393-8 - Biochar applied in the removal of emerging contaminants present in wastewater |
| Grantee: | Paulo Clairmont Feitosa de Lima Gomes |
| Support type: | Regular Research Grants |
| FAPESP's process: | 15/22828-6 - Pushing the boundaries of optical fibers: from photonics to optogenetics and environmental monitoring |
| Grantee: | Younes Messaddeq |
| Support type: | Research Projects - SPEC Program |
| FAPESP's process: | 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass |
| Grantee: | Edgar Dutra Zanotto |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |