Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor

Author(s)

Lamarca, Rafaela Silva ^{[1, 2]} ; Dorledo de Faria, Ricardo Adriano ^{[1, 3]} ; Boldrin Zanoni, Maria Valnice ^[2] ; Nalin, Marcelo ^[4] ; Feitosa de Lima Gomes, Paulo Clairmont ^[2] ; Messaddeq, Younes ^{[4, 1]}

Affiliation

^[1] Univ Laval, COPL, Quebec City, PQ G1V0A6 - Canada

^[2] Sao Paulo State Univ, Natl Inst Alternat Technol Detect Toxicol Evaluat, UNESP, Inst Chem, POB 355, BR-14800900 Araraquara, SP - Brazil

^[3] Univ Fed Minas Gerais, Dept Chem Engn, BR-31270901 Belo Horizonte, MG - Brazil

^[4] Sao Paulo State Univ UNESP, Inst Chem, POB 355, BR-14800060 Araraquara, SP - Brazil

• Document type: Journal article
• Source: RSC ADVANCES; v. 10, n. 4, p. 1838-1847, JAN 12 2020.
• Web of Science Citations: 0

Abstract

In this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Universite Laval residences (Quebec, Canada) through the load transfer resistance (R-ct) using {[}Fe(CN)(6)](3-/4-) as a redox probe, and the average CIP concentration was found to be 2.90 x 10(-4) mu g mL(-1). The observed R-ct changes presented a linear relationship from CIP concentrations of 10(-5) to 1.0 mu g mL(-1), with detection and quantification limits of 2.50 x 10(-6) and 7.90 x 10(-6) mu g mL(-1), respectively. The immunosensor presented high selectivity and repeatability, as well as a good recovery rate in wastewater samples (97%). Significant interference with other compounds was not observed. The proposed method requires only 30 mu L of sample without the use of organic solvents or preceding sample preparation and/or extraction techniques. Moreover, the method is fast: only 20 min of incubation followed by 2 min of analysis time was sufficient to obtain the CIP concentration. The method's estimated cost is U\$ 2.00 per sample. (AU)

• FAPESP's process: 18/22393-8 - Biochar applied in the removal of emerging contaminants present in wastewater
• Grantee: Paulo Clairmont Feitosa de Lima Gomes
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/22828-6 - Pushing the boundaries of optical fibers: from photonics to optogenetics and environmental monitoring
• Grantee: Younes Messaddeq
• Support Opportunities: Research Projects - SPEC Program
• FAPESP's process: 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass
• Grantee: Edgar Dutra Zanotto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC