Paracoccidioides brasiliensis 14-3-3 protein is important for virulence in a murine model

Author(s)

Marcos, Caroline Maria ^[1] ; de Oliveira, Haroldo Cesar ^[1] ; Assato, Patricia Akemi ^[1] ; de Andrade, Cleverton Roberto ^[2] ; Fusco-Almeida, Ana Marisa ^[1] ; Soares Mendes-Giannini, Maria Jose ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Dept Anal Clin, Lab Micol Clin, Campus Araraquara, Araraquara, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Fac Odontol Araraquara, Dept Fisiol & Patol, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Medical Mycology; v. 57, n. 7, p. 900-904, OCT 2019.
• Web of Science Citations: 0

Abstract

The Paracoccidioides brasiliensis strain downregulated the expression of adhesin Pb14-3-3 (Pb14-3-3 aRNA) was evaluated in a murine model of paracoccidioidomycosis (PCM). Pb14-3-3 aRNA displays attenuated virulence and triggered the formation of fewer granulomas by lowering the fungal burden in the lungs. Additionally, the Pb14-3-3 aRNA showed more elongated yeast cells and less ability to induce pneumocytes apoptosis in vitro. Our results show that 14-3-3 is an important virulence factor in P. brasiliensis-induced pulmonary infection. (AU)

• FAPESP's process: 16/17048-4 - Two-component signal transduction (TCST) system as a new target for the treatment of paracoccidioidomycosis
• Grantee: Caroline Maria Marcos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/14023-8 - Use of peptides with anti-adhesive activity in Paracoccidoides spp. in the treatment and prophylaxis of the paracoccidioidomycosis
• Grantee: Haroldo Cesar de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/03700-9 - Alternative animal as a model to study Paracoccidioides- host interaction: virulence, efficacy and toxicology of antifungal compounds and new preventive treatments
• Grantee: Maria José Soares Mendes Giannini
• Support Opportunities: Regular Research Grants