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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

New tetrodotoxin analogs in Brazilian pufferfishes tissues and microbiome

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Chimetto Tonon, Luciane A. [1] ; de Azevedo, Gustavo P. R. [2] ; Monteiro, Afif F. [1] ; Bernardi, I, Darlon ; Gubiani, Juliana R. [3] ; Ioca, Laura P. [3] ; Mattsson, Hannah K. [3] ; Moreira, Ana Paula B. [2] ; Gomes, Alexandre F. [4] ; Pires Junior, Osmindo R. [5] ; Pedrosa, Carolina da S. G. [6] ; Souza, Leticia R. Q. [6] ; Rehen, Stevens K. [7, 6] ; Thompson, Cristiane C. [2] ; Thompson, Fabiano L. [2] ; Berlinck, Roberto G. S. [3]
Total Authors: 16
[1] Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil
[2] Fed Univ Rio de Janeiro UFRJ, Inst Biol, Lab Microbiol, SAGE COPPE, Rio De Janeiro, RJ - Brazil
[3] Bernardi, Darlon, I, Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil
[4] Waters Technol Brazil, Barueri, SP - Brazil
[5] Brasilia Univ, Biol Inst, Lab Toxicol, Brasilia, DF - Brazil
[6] DOr Inst Res & Educ IDOR, Rio De Janeiro, RJ - Brazil
[7] Fed Univ Rio de Janeiro UFRJ, Inst Biomed Sci, Rio De Janeiro, RJ - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Chemosphere; v. 242, MAR 2020.
Web of Science Citations: 0

While tetrodotoxin (TTX) is commonly found in pufferfish tissues, it is unclear if bacterial symbionts isolated from pufferfish tissues can produce TTX. In this investigation, UPLC qTOE-MS/MS analysis of tissue extracts obtained from Sphoeroides spengleri and Canthigaster figuereidoi identified TTX in their composition, indicating their consumption is unsafe. UPLC qTOE-MS/MS analysis coupled with Molecular Networking indicated new TTX analogs (methyl-TTX, TTX-acetate, hydroxypropyl-TTX and glycerol-TTX). Bacterial extracts from sixteen strains revealed a compound with a {[}M+H](+) ion at m/z 320.1088, identical to TTX. However, TTX itself was not detected in these cultures by UPLC-MS/MS. Neurotoxicity of Vibrio A665 purified fraction 2 (with precursor {[}M+H](+) ion at m/z 320.1088) was significant in human neural stem cells (hNSCs), but the Na-v blockage activity was not confirmed by the veratridine/ouabain essays, indicating a possible difference in the mechanism of action between the bacterium A665 purified fraction 2 and TTX. Vibrios symbionts of pufferfish point out involving in the production of TTX precursors. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/50228-8 - Biodiversity components, and its metabolic characters, of Brazilian Islands
Grantee:Roberto Gomes de Souza Berlinck
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 16/14375-4 - Omics tools applied to the discovery of tetrodotoxin producing organisms and biosynthetic genes
Grantee:Luciane Alessandra Chimetto Tonon
Support type: Scholarships in Brazil - Post-Doctorate