Omics tools applied to the discovery of tetrodotoxin producing organisms and biosy...
| Full text | |
| Author(s): Show less - |
Chimetto Tonon, Luciane A.
[1]
;
de Azevedo, Gustavo P. R.
[2]
;
Monteiro, Afif F.
[1]
;
Bernardi, I, Darlon
;
Gubiani, Juliana R.
[3]
;
Ioca, Laura P.
[3]
;
Mattsson, Hannah K.
[3]
;
Moreira, Ana Paula B.
[2]
;
Gomes, Alexandre F.
[4]
;
Pires Junior, Osmindo R.
[5]
;
Pedrosa, Carolina da S. G.
[6]
;
Souza, Leticia R. Q.
[6]
;
Rehen, Stevens K.
[7, 6]
;
Thompson, Cristiane C.
[2]
;
Thompson, Fabiano L.
[2]
;
Berlinck, Roberto G. S.
[3]
Total Authors: 16
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil
[2] Fed Univ Rio de Janeiro UFRJ, Inst Biol, Lab Microbiol, SAGE COPPE, Rio De Janeiro, RJ - Brazil
[3] Bernardi, Darlon, I, Univ Sao Paulo, Inst Quim Sao Carlos, CP 780, BR-13560970 Sao Carlos, SP - Brazil
[4] Waters Technol Brazil, Barueri, SP - Brazil
[5] Brasilia Univ, Biol Inst, Lab Toxicol, Brasilia, DF - Brazil
[6] DOr Inst Res & Educ IDOR, Rio De Janeiro, RJ - Brazil
[7] Fed Univ Rio de Janeiro UFRJ, Inst Biomed Sci, Rio De Janeiro, RJ - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Chemosphere; v. 242, MAR 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
While tetrodotoxin (TTX) is commonly found in pufferfish tissues, it is unclear if bacterial symbionts isolated from pufferfish tissues can produce TTX. In this investigation, UPLC qTOE-MS/MS analysis of tissue extracts obtained from Sphoeroides spengleri and Canthigaster figuereidoi identified TTX in their composition, indicating their consumption is unsafe. UPLC qTOE-MS/MS analysis coupled with Molecular Networking indicated new TTX analogs (methyl-TTX, TTX-acetate, hydroxypropyl-TTX and glycerol-TTX). Bacterial extracts from sixteen strains revealed a compound with a {[}M+H](+) ion at m/z 320.1088, identical to TTX. However, TTX itself was not detected in these cultures by UPLC-MS/MS. Neurotoxicity of Vibrio A665 purified fraction 2 (with precursor {[}M+H](+) ion at m/z 320.1088) was significant in human neural stem cells (hNSCs), but the Na-v blockage activity was not confirmed by the veratridine/ouabain essays, indicating a possible difference in the mechanism of action between the bacterium A665 purified fraction 2 and TTX. Vibrios symbionts of pufferfish point out involving in the production of TTX precursors. (C) 2019 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 13/50228-8 - Biodiversity components, and its metabolic characters, of Brazilian Islands |
| Grantee: | Roberto Gomes de Souza Berlinck |
| Support type: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 16/14375-4 - Omics tools applied to the discovery of tetrodotoxin producing organisms and biosynthetic genes |
| Grantee: | Luciane Alessandra Chimetto Tonon |
| Support type: | Scholarships in Brazil - Post-Doctorate |