New Detector Based on Composite of Carbon Nanotubes with Nanoparticles of Cobalt Oxide for Carbohydrates Analysis by HPLC with Reverse Pulsed Amperometric Detection

Author(s)

de Sa, Acelino C. ^[1] ; Paim, Leonardo L. ^[2] ; Stradiotto, Nelson R. ^{[3, 1, 4]}

Affiliation

^[1] Univ Estadual Paulista, Inst Quim, UNESP, BR-14800060 Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, Campus Expt Rosana, UNESP, BR-19273000 Rosana, SP - Brazil

^[3] Univ Fed Fluminense, EEIMVR, BR-27255125 Volta Redonda, RJ - Brazil

^[4] Univ Estadual Paulista, Inst Pesquisa Bioenergia, UNESP, BR-13500230 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 31, n. 3, p. 456-466, MAR 2020.
• Web of Science Citations: 0

Abstract

This work aims to present an alternative detector for the determination of arabinose, galactose, glucose and xylose in hydrolyzed sugarcane bagasse. The detector was developed using an electrode modified with multi-walled carbon nanotubes containing cobalt oxide nanoparticles (GCE/MWCNT/CoOOH) applied in high-performance liquid chromatography (HPLC) with reverse pulsed amperometric detection (RPAD). The limits of detection obtained were 3.4 × 10-6, 4.4 × 10-6, 3.6 × 10-6 and 5.0 × 10-6 mol L-1 for arabinose, galactose, glucose and xylose, respectively. The standard addition method was used to determine the concentration of sugars in the hydrolyzed sugarcane. The determined concentrations for arabinose, galactose, glucose, and xylose were 1.0 × 10-3, 7.2 × 10-5, 3.0 × 10-3 and 2.4 × 10-3 mol L-1, respectively. The results demonstrate that this new method can be used for the detection of sugars without the interference of other electroactive species. (AU)

• FAPESP's process: 11/19289-5 - Determination of sugar in bagasse and straw of cane sugar by high performance liquid chromatography with electrochemical detection using electrode modified carbon nanotubes with metal oxides
• Grantee: Acelino Cardoso de Sá
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/15557-3 - Determination of carbohydrates in sugarcane bagasse employing a voltammetric electronic tongue formed by nickel oxy-hydroxide/MWCNT modified electrodes
• Grantee: Acelino Cardoso de Sá
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 12/00258-5 - Development of new methods for determination of sugars, furanic aldehydes and ironic acids from lignocellulosic materials derivatives of cane sugar
• Grantee: Nelson Ramos Stradiotto
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants