| Full text | |
| Author(s): |
Ribeiro, Giulia M.
[1]
;
Porfirio-Sousa, Alfredo L.
[1]
;
Maurer-Alcala, Xyrus X.
[2, 3, 4]
;
Katz, Laura A.
[2]
;
Lahr, Daniel J. G.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biosci, Dept Zool, Matao St, Travessa 14 Cidade Univ, BR-05508090 Sao Paulo, SP - Brazil
[2] Smith Coll, Dept Biol Sci, 10 Elm St, Northampton, MA 01063 - USA
[3] Univ Massachussetts, Program Organism & Evolutionary Biol, 230 Stockbridge Rd, Amherst, MA 01002 - USA
[4] Univ Bern, Inst Cell Biol, Baltzerstr 4, CH-3012 Bern - Switzerland
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of Eukaryotic Microbiology; v. 67, n. 3 FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Arcella, a diverse understudied genus of testate amoebae is a member of Tubulinea in Amoebozoa group. Transcriptomes are a powerful tool for characterization of these organisms as they are an efficient way of characterizing the protein-coding potential of the genome. In this work, we employed both single-cell and clonal populations transcriptomics to create a reference transcriptome for Arcella. We compared our results with annotations of Dictyostelium discoideum, a model Amoebozoan. We assembled a pool of 38 Arcella intermedia transcriptomes, which after filtering are composed of a total of 14,712 translated proteins. There are GO categories enriched in Arcella including mainly intracellular signal transduction pathways; we also used KEGG to annotate 11,546 contigs, which also have similar distribution to Dictyostelium. A large portion of data is still impossible to assign to a gene family, probably due to a combination of lineage-specific genes, incomplete sequences in the transcriptome and rapidly evolved genes. Some absences in pathways could also be related to low expression of these genes. We provide a reference database for Arcella, and we highlight the emergence of the need for further gene discovery in Arcella. (AU) | |
| FAPESP's process: | 17/16077-3 - Energetic metabolism analysis along culture growth of ARCELLA intermedia |
| Grantee: | Giulia Magri Ribeiro |
| Support type: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 16/14317-4 - Identification of genes involved in the test formation in Arcella intermedia leavis through transcriptome |
| Grantee: | Alfredo Leonardo Porfirio de Sousa |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/14402-1 - Analysis of Arcella vulgaris expression profile along your growth curve in laboratory |
| Grantee: | Giulia Magri Ribeiro |
| Support type: | Scholarships in Brazil - Master |